Protocol for measuring VOC biotransformation rates

Jeff R. Kemp, John Bell, Wayne Parker, Bruce Rittmann, Doug Thompson, Richard G. Zytner

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A protocol has been developed to determine kinetic parameters for the biotransformation of volatile organic compounds (VOCs). The protocol employs a batch system with zero headspace and utilizes a bubbleless oxygenation system to minimize losses due to volatilization. The reactor is filled with a suitable wastewater solution, primary clarified effluent or a synthetic mixture, and a volume of active biomass. It is then dosed with a solution of VOCs. The VOC biotransformation rates are determined by withdrawing samples from the reactor over a period of four to eight hours. Abiotic experiments have been completed to confirm that losses due to sorption and volatilization are negligible. Experiments have also been completed to verify that biotransformation rates observed with the protocol reactor accurately predict those observed in full scale operations. The protocol has been used to examine effects of varying the chemical oxygen demand (COD) concentration of the primary substrate and the dissolved oxygen (DO) concentration in the bioreactor under aerobic conditions. Primary substrate COD concentrations of 20, 100 and 500 mg/L and DO concentrations of 0.5 and 5.0 mg/L were selected. The effects of varying the primary substrate COD concentration in anaerobic and anoxic environments were also examined. Significant differences in biotransformation rates were observed under aerobic conditions when the COD and DO concentrations in the bioreactor were changed. It was found that biotransformation kinetic parameters varied by up to an order of magnitude for certain compounds. The highest biotransformation rates were observed under low COD, high DO conditions. No significant effects were found for the anaerobic and anoxic environments. This study will help provide a better understanding of VOC biotransformation in municipal and industrial wastewater treatment works. Results from this study can be utilized by predictive fate models such as WATER8 or TOXCHEM.

Original languageEnglish (US)
Title of host publicationProceedings of the Air & Waste Management Association's Annual Meeting & Exhibition
Editors Anon
Place of PublicationPittsburgh, PA, United States
PublisherAir & Waste Management Assoc
StatePublished - 1996
Externally publishedYes
EventProceedings of the 1996 Air & Waste Management Association's 89th Annual Meeting & Exhibition - Nashville, TN, USA
Duration: Jun 23 1996Jun 28 1996

Other

OtherProceedings of the 1996 Air & Waste Management Association's 89th Annual Meeting & Exhibition
CityNashville, TN, USA
Period6/23/966/28/96

Fingerprint

Volatile organic compounds
Chemical oxygen demand
Dissolved oxygen
Bioreactors
Vaporization
Kinetic parameters
Substrates
Oxygenation
Biotransformation
Wastewater treatment
Sorption
Effluents
Biomass
Wastewater
Experiments

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kemp, J. R., Bell, J., Parker, W., Rittmann, B., Thompson, D., & Zytner, R. G. (1996). Protocol for measuring VOC biotransformation rates. In Anon (Ed.), Proceedings of the Air & Waste Management Association's Annual Meeting & Exhibition Pittsburgh, PA, United States: Air & Waste Management Assoc.

Protocol for measuring VOC biotransformation rates. / Kemp, Jeff R.; Bell, John; Parker, Wayne; Rittmann, Bruce; Thompson, Doug; Zytner, Richard G.

Proceedings of the Air & Waste Management Association's Annual Meeting & Exhibition. ed. / Anon. Pittsburgh, PA, United States : Air & Waste Management Assoc, 1996.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kemp, JR, Bell, J, Parker, W, Rittmann, B, Thompson, D & Zytner, RG 1996, Protocol for measuring VOC biotransformation rates. in Anon (ed.), Proceedings of the Air & Waste Management Association's Annual Meeting & Exhibition. Air & Waste Management Assoc, Pittsburgh, PA, United States, Proceedings of the 1996 Air & Waste Management Association's 89th Annual Meeting & Exhibition, Nashville, TN, USA, 6/23/96.
Kemp JR, Bell J, Parker W, Rittmann B, Thompson D, Zytner RG. Protocol for measuring VOC biotransformation rates. In Anon, editor, Proceedings of the Air & Waste Management Association's Annual Meeting & Exhibition. Pittsburgh, PA, United States: Air & Waste Management Assoc. 1996
Kemp, Jeff R. ; Bell, John ; Parker, Wayne ; Rittmann, Bruce ; Thompson, Doug ; Zytner, Richard G. / Protocol for measuring VOC biotransformation rates. Proceedings of the Air & Waste Management Association's Annual Meeting & Exhibition. editor / Anon. Pittsburgh, PA, United States : Air & Waste Management Assoc, 1996.
@inproceedings{7783a967aa6549cb807a8ee36d1b59e7,
title = "Protocol for measuring VOC biotransformation rates",
abstract = "A protocol has been developed to determine kinetic parameters for the biotransformation of volatile organic compounds (VOCs). The protocol employs a batch system with zero headspace and utilizes a bubbleless oxygenation system to minimize losses due to volatilization. The reactor is filled with a suitable wastewater solution, primary clarified effluent or a synthetic mixture, and a volume of active biomass. It is then dosed with a solution of VOCs. The VOC biotransformation rates are determined by withdrawing samples from the reactor over a period of four to eight hours. Abiotic experiments have been completed to confirm that losses due to sorption and volatilization are negligible. Experiments have also been completed to verify that biotransformation rates observed with the protocol reactor accurately predict those observed in full scale operations. The protocol has been used to examine effects of varying the chemical oxygen demand (COD) concentration of the primary substrate and the dissolved oxygen (DO) concentration in the bioreactor under aerobic conditions. Primary substrate COD concentrations of 20, 100 and 500 mg/L and DO concentrations of 0.5 and 5.0 mg/L were selected. The effects of varying the primary substrate COD concentration in anaerobic and anoxic environments were also examined. Significant differences in biotransformation rates were observed under aerobic conditions when the COD and DO concentrations in the bioreactor were changed. It was found that biotransformation kinetic parameters varied by up to an order of magnitude for certain compounds. The highest biotransformation rates were observed under low COD, high DO conditions. No significant effects were found for the anaerobic and anoxic environments. This study will help provide a better understanding of VOC biotransformation in municipal and industrial wastewater treatment works. Results from this study can be utilized by predictive fate models such as WATER8 or TOXCHEM.",
author = "Kemp, {Jeff R.} and John Bell and Wayne Parker and Bruce Rittmann and Doug Thompson and Zytner, {Richard G.}",
year = "1996",
language = "English (US)",
editor = "Anon",
booktitle = "Proceedings of the Air & Waste Management Association's Annual Meeting & Exhibition",
publisher = "Air & Waste Management Assoc",

}

TY - GEN

T1 - Protocol for measuring VOC biotransformation rates

AU - Kemp, Jeff R.

AU - Bell, John

AU - Parker, Wayne

AU - Rittmann, Bruce

AU - Thompson, Doug

AU - Zytner, Richard G.

PY - 1996

Y1 - 1996

N2 - A protocol has been developed to determine kinetic parameters for the biotransformation of volatile organic compounds (VOCs). The protocol employs a batch system with zero headspace and utilizes a bubbleless oxygenation system to minimize losses due to volatilization. The reactor is filled with a suitable wastewater solution, primary clarified effluent or a synthetic mixture, and a volume of active biomass. It is then dosed with a solution of VOCs. The VOC biotransformation rates are determined by withdrawing samples from the reactor over a period of four to eight hours. Abiotic experiments have been completed to confirm that losses due to sorption and volatilization are negligible. Experiments have also been completed to verify that biotransformation rates observed with the protocol reactor accurately predict those observed in full scale operations. The protocol has been used to examine effects of varying the chemical oxygen demand (COD) concentration of the primary substrate and the dissolved oxygen (DO) concentration in the bioreactor under aerobic conditions. Primary substrate COD concentrations of 20, 100 and 500 mg/L and DO concentrations of 0.5 and 5.0 mg/L were selected. The effects of varying the primary substrate COD concentration in anaerobic and anoxic environments were also examined. Significant differences in biotransformation rates were observed under aerobic conditions when the COD and DO concentrations in the bioreactor were changed. It was found that biotransformation kinetic parameters varied by up to an order of magnitude for certain compounds. The highest biotransformation rates were observed under low COD, high DO conditions. No significant effects were found for the anaerobic and anoxic environments. This study will help provide a better understanding of VOC biotransformation in municipal and industrial wastewater treatment works. Results from this study can be utilized by predictive fate models such as WATER8 or TOXCHEM.

AB - A protocol has been developed to determine kinetic parameters for the biotransformation of volatile organic compounds (VOCs). The protocol employs a batch system with zero headspace and utilizes a bubbleless oxygenation system to minimize losses due to volatilization. The reactor is filled with a suitable wastewater solution, primary clarified effluent or a synthetic mixture, and a volume of active biomass. It is then dosed with a solution of VOCs. The VOC biotransformation rates are determined by withdrawing samples from the reactor over a period of four to eight hours. Abiotic experiments have been completed to confirm that losses due to sorption and volatilization are negligible. Experiments have also been completed to verify that biotransformation rates observed with the protocol reactor accurately predict those observed in full scale operations. The protocol has been used to examine effects of varying the chemical oxygen demand (COD) concentration of the primary substrate and the dissolved oxygen (DO) concentration in the bioreactor under aerobic conditions. Primary substrate COD concentrations of 20, 100 and 500 mg/L and DO concentrations of 0.5 and 5.0 mg/L were selected. The effects of varying the primary substrate COD concentration in anaerobic and anoxic environments were also examined. Significant differences in biotransformation rates were observed under aerobic conditions when the COD and DO concentrations in the bioreactor were changed. It was found that biotransformation kinetic parameters varied by up to an order of magnitude for certain compounds. The highest biotransformation rates were observed under low COD, high DO conditions. No significant effects were found for the anaerobic and anoxic environments. This study will help provide a better understanding of VOC biotransformation in municipal and industrial wastewater treatment works. Results from this study can be utilized by predictive fate models such as WATER8 or TOXCHEM.

UR - http://www.scopus.com/inward/record.url?scp=0030314612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030314612&partnerID=8YFLogxK

M3 - Conference contribution

BT - Proceedings of the Air & Waste Management Association's Annual Meeting & Exhibition

A2 - Anon, null

PB - Air & Waste Management Assoc

CY - Pittsburgh, PA, United States

ER -