Protocol for electrical modulation of ERK signaling dynamics in cell lines

Kan Zhu, Liang Guo, Preena Patel, John Albeck, Quan Qing, Wolfgang Losert, Min Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real time without chemical agents or gene disruption. ERK activation is assessed by comparing the relative intensity of nuclear fluorescence to cytosolic fluorescence in live-cell conditions. The approach can be applied to other signaling pathways as well. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).

Original languageEnglish (US)
Article number101752
JournalSTAR Protocols
Volume3
Issue number4
DOIs
StatePublished - Dec 16 2022

Keywords

  • Biotechnology and bioengineering
  • Cell biology
  • Cell culture
  • Microscopy
  • Signal transduction

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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