Prothrombin levels are increased in the estrogen- treated immature rat uterus

Katherine P. Henrikson, Elena E. Jazin, Jeffrey A. Greenwood, Herbert W. Dickerman, Katherine P. Henrikson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

An estrogen-responsive uterine proenzyme of a proteinase in the immature rat uterus has been known for some time. Its mol wt is 77, 000, its N-terminal amino acid sequence is the same as prothrombin’s for 15 residues, it contains γ- carboxyl glutamate residues, its biosynthesis is prevented by warfarin, it cross-reacts with antibodies to human and rat prothrombin, and it can be activated by human factor Xa or a uterine procoagulant. The products of activation, when separated on sodium dodecyl sulfate-gels, react with antibodies to human or rat prothrombin to give bands that have mol wt corresponding to those of the products of activation of prothrombin. These activation intermediates hydrolyze synthetic substrates specific for thrombin and have the same mol wt as the activation products of prothrombin. The proteinase generated in the activation has the following properties of thrombin: It is inhibited by hirudin and PheProArg-chloromethyl ketone, it has kinetic constants similar to those of thrombin with tripeptide p- nitroanilides as substrates, and it digests actin to give the same peptides as thrombin. We conclude that the uterine proenzyme is prothrombin. The time course of the prothrombin response to estrogen suggests that prothrombin enters the uterus as part of the transudation of plasma proteins that occurs after estrogen stim-ulation. A membrane-bound uterine procoagulant that activates uterine prothrombin also increases in response to estrogen stim-ulation. We propose that the simultaneous increase in these two activities results in a localized generation of thrombin, a well characterized mitogen in fibroblasts and epithelial cells. Our results suggest that thrombin may have a vital function as a mitogen in the early steps of the estrogen-stimulated hypertrophy and hyperplasia of the immature uterus.

Original languageEnglish (US)
Pages (from-to)167-175
Number of pages9
JournalEndocrinology
Volume126
Issue number1
DOIs
StatePublished - Jan 1990
Externally publishedYes

Fingerprint

Prothrombin
Uterus
Estrogens
Thrombin
Enzyme Precursors
Mitogens
Peptide Hydrolases
Hirudins
Factor Xa
Antibodies
Prothrombin Time
Warfarin
Ketones
Sodium Dodecyl Sulfate
Hypertrophy
Hyperplasia
Actins
Blood Proteins
Glutamic Acid
Amino Acid Sequence

ASJC Scopus subject areas

  • Endocrinology

Cite this

Henrikson, K. P., Jazin, E. E., Greenwood, J. A., Dickerman, H. W., & Henrikson, K. P. (1990). Prothrombin levels are increased in the estrogen- treated immature rat uterus. Endocrinology, 126(1), 167-175. https://doi.org/10.1210/endo-126-1-167

Prothrombin levels are increased in the estrogen- treated immature rat uterus. / Henrikson, Katherine P.; Jazin, Elena E.; Greenwood, Jeffrey A.; Dickerman, Herbert W.; Henrikson, Katherine P.

In: Endocrinology, Vol. 126, No. 1, 01.1990, p. 167-175.

Research output: Contribution to journalArticle

Henrikson, KP, Jazin, EE, Greenwood, JA, Dickerman, HW & Henrikson, KP 1990, 'Prothrombin levels are increased in the estrogen- treated immature rat uterus', Endocrinology, vol. 126, no. 1, pp. 167-175. https://doi.org/10.1210/endo-126-1-167
Henrikson, Katherine P. ; Jazin, Elena E. ; Greenwood, Jeffrey A. ; Dickerman, Herbert W. ; Henrikson, Katherine P. / Prothrombin levels are increased in the estrogen- treated immature rat uterus. In: Endocrinology. 1990 ; Vol. 126, No. 1. pp. 167-175.
@article{e3fa3709b01d4d6daeb0a786a66f5a37,
title = "Prothrombin levels are increased in the estrogen- treated immature rat uterus",
abstract = "An estrogen-responsive uterine proenzyme of a proteinase in the immature rat uterus has been known for some time. Its mol wt is 77, 000, its N-terminal amino acid sequence is the same as prothrombin’s for 15 residues, it contains γ- carboxyl glutamate residues, its biosynthesis is prevented by warfarin, it cross-reacts with antibodies to human and rat prothrombin, and it can be activated by human factor Xa or a uterine procoagulant. The products of activation, when separated on sodium dodecyl sulfate-gels, react with antibodies to human or rat prothrombin to give bands that have mol wt corresponding to those of the products of activation of prothrombin. These activation intermediates hydrolyze synthetic substrates specific for thrombin and have the same mol wt as the activation products of prothrombin. The proteinase generated in the activation has the following properties of thrombin: It is inhibited by hirudin and PheProArg-chloromethyl ketone, it has kinetic constants similar to those of thrombin with tripeptide p- nitroanilides as substrates, and it digests actin to give the same peptides as thrombin. We conclude that the uterine proenzyme is prothrombin. The time course of the prothrombin response to estrogen suggests that prothrombin enters the uterus as part of the transudation of plasma proteins that occurs after estrogen stim-ulation. A membrane-bound uterine procoagulant that activates uterine prothrombin also increases in response to estrogen stim-ulation. We propose that the simultaneous increase in these two activities results in a localized generation of thrombin, a well characterized mitogen in fibroblasts and epithelial cells. Our results suggest that thrombin may have a vital function as a mitogen in the early steps of the estrogen-stimulated hypertrophy and hyperplasia of the immature uterus.",
author = "Henrikson, {Katherine P.} and Jazin, {Elena E.} and Greenwood, {Jeffrey A.} and Dickerman, {Herbert W.} and Henrikson, {Katherine P.}",
year = "1990",
month = "1",
doi = "10.1210/endo-126-1-167",
language = "English (US)",
volume = "126",
pages = "167--175",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "1",

}

TY - JOUR

T1 - Prothrombin levels are increased in the estrogen- treated immature rat uterus

AU - Henrikson, Katherine P.

AU - Jazin, Elena E.

AU - Greenwood, Jeffrey A.

AU - Dickerman, Herbert W.

AU - Henrikson, Katherine P.

PY - 1990/1

Y1 - 1990/1

N2 - An estrogen-responsive uterine proenzyme of a proteinase in the immature rat uterus has been known for some time. Its mol wt is 77, 000, its N-terminal amino acid sequence is the same as prothrombin’s for 15 residues, it contains γ- carboxyl glutamate residues, its biosynthesis is prevented by warfarin, it cross-reacts with antibodies to human and rat prothrombin, and it can be activated by human factor Xa or a uterine procoagulant. The products of activation, when separated on sodium dodecyl sulfate-gels, react with antibodies to human or rat prothrombin to give bands that have mol wt corresponding to those of the products of activation of prothrombin. These activation intermediates hydrolyze synthetic substrates specific for thrombin and have the same mol wt as the activation products of prothrombin. The proteinase generated in the activation has the following properties of thrombin: It is inhibited by hirudin and PheProArg-chloromethyl ketone, it has kinetic constants similar to those of thrombin with tripeptide p- nitroanilides as substrates, and it digests actin to give the same peptides as thrombin. We conclude that the uterine proenzyme is prothrombin. The time course of the prothrombin response to estrogen suggests that prothrombin enters the uterus as part of the transudation of plasma proteins that occurs after estrogen stim-ulation. A membrane-bound uterine procoagulant that activates uterine prothrombin also increases in response to estrogen stim-ulation. We propose that the simultaneous increase in these two activities results in a localized generation of thrombin, a well characterized mitogen in fibroblasts and epithelial cells. Our results suggest that thrombin may have a vital function as a mitogen in the early steps of the estrogen-stimulated hypertrophy and hyperplasia of the immature uterus.

AB - An estrogen-responsive uterine proenzyme of a proteinase in the immature rat uterus has been known for some time. Its mol wt is 77, 000, its N-terminal amino acid sequence is the same as prothrombin’s for 15 residues, it contains γ- carboxyl glutamate residues, its biosynthesis is prevented by warfarin, it cross-reacts with antibodies to human and rat prothrombin, and it can be activated by human factor Xa or a uterine procoagulant. The products of activation, when separated on sodium dodecyl sulfate-gels, react with antibodies to human or rat prothrombin to give bands that have mol wt corresponding to those of the products of activation of prothrombin. These activation intermediates hydrolyze synthetic substrates specific for thrombin and have the same mol wt as the activation products of prothrombin. The proteinase generated in the activation has the following properties of thrombin: It is inhibited by hirudin and PheProArg-chloromethyl ketone, it has kinetic constants similar to those of thrombin with tripeptide p- nitroanilides as substrates, and it digests actin to give the same peptides as thrombin. We conclude that the uterine proenzyme is prothrombin. The time course of the prothrombin response to estrogen suggests that prothrombin enters the uterus as part of the transudation of plasma proteins that occurs after estrogen stim-ulation. A membrane-bound uterine procoagulant that activates uterine prothrombin also increases in response to estrogen stim-ulation. We propose that the simultaneous increase in these two activities results in a localized generation of thrombin, a well characterized mitogen in fibroblasts and epithelial cells. Our results suggest that thrombin may have a vital function as a mitogen in the early steps of the estrogen-stimulated hypertrophy and hyperplasia of the immature uterus.

UR - http://www.scopus.com/inward/record.url?scp=0025058605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025058605&partnerID=8YFLogxK

U2 - 10.1210/endo-126-1-167

DO - 10.1210/endo-126-1-167

M3 - Article

C2 - 2293980

AN - SCOPUS:0025058605

VL - 126

SP - 167

EP - 175

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 1

ER -