Proteomics of human urine

Visith Thongboonkerd, Pedro R. Cutillas, Robert J. Unwin, Stefan Schaub, Peter Nickerson, Marion Haubitz, Harald Mischak, Dobrin Nedelkov, Urban A. Kiernan, Randall W. Nelson

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Urine is one of the most interesting and useful human body fluids for clinical proteomics studies because of its availability in almost all patients and ease of collection without any invasive or painful procedure. Proteomic analysis of human urine offers invaluable information not only for a better understanding of the normal renal physiology and pathophysiology of kidney/kidney-related diseases, but also for biomarker discovery. This chapter provides principles, brief methods, and applications of various proteomic technologies (i.e., gel-based method, liquid chromatographybased approach, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, capillary electrophoresis coupled to mass spectrometry, and mass spectrometric immunoassay) to examine the human urine. At the end, perspectives and future directions in the field of urinary proteomics are discussed.

Original languageEnglish (US)
Title of host publicationProteomics of Human Body Fluids
Subtitle of host publicationPrinciples, Methods, and Applications
PublisherHumana Press Inc.
Pages225-268
Number of pages44
ISBN (Electronic)9781597454322
ISBN (Print)9781588296573
DOIs
StatePublished - 2007

Keywords

  • 2D-PAGE
  • SELDI
  • Urine
  • biomarker discovery
  • capillary electrophoresis
  • kidney
  • liquid chromatography
  • mass spectrometric immunoassay
  • pathophysiology
  • proteome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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