Proteomic monitoring of B cell immunity

Radwa Ewaisha, Karen Anderson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Immune monitoring is critical in settings of infection, autoimmunity, and cancer, but our understanding of the diversity of the antibody immune repertoire has been limited to selected target antigens and epitopes. Development of new vaccines requires monitoring of B cell immunity and identification of candidate antigens. As vaccines become more complex, novel techniques are required for monitoring the diversity of the B cell immune response. Since antibodies recognize both linear and conformational protein and glycoprotein epitopes, recent advances in proteomic and glycomic technologies for rapid display of antigenic structures are leading to methods for proteome-wide immune monitoring. Here, we review different approaches for protein display for immune monitoring, and provide methods for in situ protein display for the rapid detection and validation of antibody repertoires.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages131-152
Number of pages22
Volume1403
DOIs
StatePublished - Apr 1 2016

Publication series

NameMethods in Molecular Biology
Volume1403
ISSN (Print)10643745

Fingerprint

Immunologic Monitoring
Proteomics
Immunity
B-Lymphocytes
Epitopes
Vaccines
Antibody Diversity
Glycomics
Antigens
Proteins
Antibodies
Proteome
Autoimmunity
Glycoproteins
Technology
Infection
Neoplasms

Keywords

  • Antibody
  • B cell
  • Epitope
  • Immune monitoring
  • Immunity
  • Phage display
  • Protein arrays
  • Protein display

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ewaisha, R., & Anderson, K. (2016). Proteomic monitoring of B cell immunity. In Methods in Molecular Biology (Vol. 1403, pp. 131-152). (Methods in Molecular Biology; Vol. 1403). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3387-7_6

Proteomic monitoring of B cell immunity. / Ewaisha, Radwa; Anderson, Karen.

Methods in Molecular Biology. Vol. 1403 Humana Press Inc., 2016. p. 131-152 (Methods in Molecular Biology; Vol. 1403).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ewaisha, R & Anderson, K 2016, Proteomic monitoring of B cell immunity. in Methods in Molecular Biology. vol. 1403, Methods in Molecular Biology, vol. 1403, Humana Press Inc., pp. 131-152. https://doi.org/10.1007/978-1-4939-3387-7_6
Ewaisha R, Anderson K. Proteomic monitoring of B cell immunity. In Methods in Molecular Biology. Vol. 1403. Humana Press Inc. 2016. p. 131-152. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3387-7_6
Ewaisha, Radwa ; Anderson, Karen. / Proteomic monitoring of B cell immunity. Methods in Molecular Biology. Vol. 1403 Humana Press Inc., 2016. pp. 131-152 (Methods in Molecular Biology).
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