Proteomic monitoring of B cell immunity

Radwa Ewaisha, Karen Anderson

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Immune monitoring is critical in settings of infection, autoimmunity, and cancer, but our understanding of the diversity of the antibody immune repertoire has been limited to selected target antigens and epitopes. Development of new vaccines requires monitoring of B cell immunity and identification of candidate antigens. As vaccines become more complex, novel techniques are required for monitoring the diversity of the B cell immune response. Since antibodies recognize both linear and conformational protein and glycoprotein epitopes, recent advances in proteomic and glycomic technologies for rapid display of antigenic structures are leading to methods for proteome-wide immune monitoring. Here, we review different approaches for protein display for immune monitoring, and provide methods for in situ protein display for the rapid detection and validation of antibody repertoires.

    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages131-152
    Number of pages22
    Volume1403
    DOIs
    StatePublished - Apr 1 2016

    Publication series

    NameMethods in Molecular Biology
    Volume1403
    ISSN (Print)10643745

    Fingerprint

    Immunologic Monitoring
    Proteomics
    Immunity
    B-Lymphocytes
    Epitopes
    Vaccines
    Antibody Diversity
    Glycomics
    Antigens
    Proteins
    Antibodies
    Proteome
    Autoimmunity
    Glycoproteins
    Technology
    Infection
    Neoplasms

    Keywords

    • Antibody
    • B cell
    • Epitope
    • Immune monitoring
    • Immunity
    • Phage display
    • Protein arrays
    • Protein display

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

    Cite this

    Ewaisha, R., & Anderson, K. (2016). Proteomic monitoring of B cell immunity. In Methods in Molecular Biology (Vol. 1403, pp. 131-152). (Methods in Molecular Biology; Vol. 1403). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3387-7_6

    Proteomic monitoring of B cell immunity. / Ewaisha, Radwa; Anderson, Karen.

    Methods in Molecular Biology. Vol. 1403 Humana Press Inc., 2016. p. 131-152 (Methods in Molecular Biology; Vol. 1403).

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Ewaisha, R & Anderson, K 2016, Proteomic monitoring of B cell immunity. in Methods in Molecular Biology. vol. 1403, Methods in Molecular Biology, vol. 1403, Humana Press Inc., pp. 131-152. https://doi.org/10.1007/978-1-4939-3387-7_6
    Ewaisha R, Anderson K. Proteomic monitoring of B cell immunity. In Methods in Molecular Biology. Vol. 1403. Humana Press Inc. 2016. p. 131-152. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3387-7_6
    Ewaisha, Radwa ; Anderson, Karen. / Proteomic monitoring of B cell immunity. Methods in Molecular Biology. Vol. 1403 Humana Press Inc., 2016. pp. 131-152 (Methods in Molecular Biology).
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