Proteomic analysis of rutin-induced secreted proteins from Aspergillus flavus

Martha L. Medina, Urban A. Kiernan, Wilson A. Francisco

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Few studies have been conducted to identify the extracellular proteins and enzymes secreted by filamentous fungi, particularly with respect to dispensable metabolic pathways. Proteomic analysis has proven to be the most powerful method for identification of proteins in complex mixtures and is suitable for the study of the alteration of protein expression under different environmental conditions. The filamentous fungus Aspergillus flavus can degrade the flavonoid rutin as the only source of carbon via an extracellular enzyme system. In this study, a proteomic analysis was used to differentiate and identify the extracellular rutin-induced and non-induced proteins secreted by A. flavus. The secreted proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. While 15 rutin-induced proteins and 7 non-induced proteins were identified, more than 90 protein spots remain unidentified, indicating that these proteins are either novel proteins or proteins that have not yet been sequenced.

Original languageEnglish (US)
Pages (from-to)327-335
Number of pages9
JournalFungal Genetics and Biology
Volume41
Issue number3
DOIs
StatePublished - Mar 2004

Fingerprint

Aspergillus flavus
Rutin
Proteomics
Proteins
Fungi
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Enzymes
Metabolic Networks and Pathways
Complex Mixtures
Flavonoids
Electrophoresis
Mass Spectrometry
Carbon

Keywords

  • Aspergillus flavus
  • Dispensable metabolic pathways
  • MALDI-TOF MS
  • Proteomics
  • Rutin degradation
  • Secreted proteins
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

Proteomic analysis of rutin-induced secreted proteins from Aspergillus flavus. / Medina, Martha L.; Kiernan, Urban A.; Francisco, Wilson A.

In: Fungal Genetics and Biology, Vol. 41, No. 3, 03.2004, p. 327-335.

Research output: Contribution to journalArticle

Medina, Martha L. ; Kiernan, Urban A. ; Francisco, Wilson A. / Proteomic analysis of rutin-induced secreted proteins from Aspergillus flavus. In: Fungal Genetics and Biology. 2004 ; Vol. 41, No. 3. pp. 327-335.
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