Protein structure determination by MicroED

Brent L. Nannenga; Tamir Gonen

doi:10.1016/j.sbi.2014.03.004

Protein structure determination by MicroED

Brent L. Nannenga, Tamir Gonen

Research output: Contribution to journal › Review article › peer-review

34 Scopus citations

Abstract

In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9. Å resolution. As the method further matures, MicroED promises to offer a unique and widely applicable approach to protein crystallography using nanocrystals.

Original language	English (US)
Pages (from-to)	24-31
Number of pages	8
Journal	Current Opinion in Structural Biology
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/j.sbi.2014.03.004
State	Published - Aug 2014
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/j.sbi.2014.03.004

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title = "Protein structure determination by MicroED",

abstract = "In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9. {\AA} resolution. As the method further matures, MicroED promises to offer a unique and widely applicable approach to protein crystallography using nanocrystals.",

author = "Nannenga, {Brent L.} and Tamir Gonen",

note = "Funding Information: We thank Dan Shi and Matthew G. Iadanza (JFRC) helpful insights and discussion. This work is supported by the Howard Hughes Medical Institute.",

year = "2014",

month = aug,

doi = "10.1016/j.sbi.2014.03.004",

language = "English (US)",

volume = "27",

pages = "24--31",

journal = "Current Opinion in Structural Biology",

issn = "0959-440X",

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AU - Nannenga, Brent L.

AU - Gonen, Tamir

N1 - Funding Information: We thank Dan Shi and Matthew G. Iadanza (JFRC) helpful insights and discussion. This work is supported by the Howard Hughes Medical Institute.

PY - 2014/8

Y1 - 2014/8

N2 - In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9. Å resolution. As the method further matures, MicroED promises to offer a unique and widely applicable approach to protein crystallography using nanocrystals.

AB - In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9. Å resolution. As the method further matures, MicroED promises to offer a unique and widely applicable approach to protein crystallography using nanocrystals.

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JO - Current Opinion in Structural Biology

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SN - 0959-440X

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Protein structure determination by MicroED

Abstract

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