Abstract
In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9. Å resolution. As the method further matures, MicroED promises to offer a unique and widely applicable approach to protein crystallography using nanocrystals.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 24-31 |
| Number of pages | 8 |
| Journal | Current Opinion in Structural Biology |
| Volume | 27 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 2014 |
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology
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Cite this
Nannenga, B. L., & Gonen, T. (2014). Protein structure determination by MicroED. Current Opinion in Structural Biology, 27(1), 24-31. https://doi.org/10.1016/j.sbi.2014.03.004