Abstract
In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9. Å resolution. As the method further matures, MicroED promises to offer a unique and widely applicable approach to protein crystallography using nanocrystals.
Original language | English (US) |
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Pages (from-to) | 24-31 |
Number of pages | 8 |
Journal | Current Opinion in Structural Biology |
Volume | 27 |
Issue number | 1 |
DOIs | |
State | Published - Aug 2014 |
Externally published | Yes |
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology