Protein structure determination by MicroED

Brent L. Nannenga, Tamir Gonen

Research output: Contribution to journalReview articlepeer-review

34 Scopus citations


In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9. Å resolution. As the method further matures, MicroED promises to offer a unique and widely applicable approach to protein crystallography using nanocrystals.

Original languageEnglish (US)
Pages (from-to)24-31
Number of pages8
JournalCurrent Opinion in Structural Biology
Issue number1
StatePublished - Aug 2014
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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