Protein-RNA interactions in the subunits of human nuclear RNase P

Taijiao Jiang; Cecilia Guerrier-Takada; Sidney Altman

doi:10.1017/S1355838201010299

Protein-RNA interactions in the subunits of human nuclear RNase P

Taijiao Jiang, Cecilia Guerrier-Takada, Sidney Altman

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

A yeast three-hybrid system was employed to analyze interactions in vivo between H1 RNA, the RNA subunit of human nuclear RNase P, and eight of the protein subunits of the enzyme. The genetic analysis indicates that subunits Rpp21, Rpp29, Rpp30, and Rpp38 interact directly with H1 RNA. The results of direct UV crosslinking studies of the purified RNase P holoenzyme confirm the results of the three-hybrid assay.

Original language	English (US)
Pages (from-to)	937-941
Number of pages	5
Journal	RNA
Volume	7
Issue number	7
DOIs	https://doi.org/10.1017/S1355838201010299
State	Published - 2001
Externally published	Yes

Keywords

HeLa cells
Precursor tRNAs
Three-hybrid assay
UV crosslinking

ASJC Scopus subject areas

Molecular Biology

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10.1017/S1355838201010299

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title = "Protein-RNA interactions in the subunits of human nuclear RNase P",

abstract = "A yeast three-hybrid system was employed to analyze interactions in vivo between H1 RNA, the RNA subunit of human nuclear RNase P, and eight of the protein subunits of the enzyme. The genetic analysis indicates that subunits Rpp21, Rpp29, Rpp30, and Rpp38 interact directly with H1 RNA. The results of direct UV crosslinking studies of the purified RNase P holoenzyme confirm the results of the three-hybrid assay.",

keywords = "HeLa cells, Precursor tRNAs, Three-hybrid assay, UV crosslinking",

author = "Taijiao Jiang and Cecilia Guerrier-Takada and Sidney Altman",

year = "2001",

doi = "10.1017/S1355838201010299",

language = "English (US)",

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pages = "937--941",

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T1 - Protein-RNA interactions in the subunits of human nuclear RNase P

AU - Jiang, Taijiao

AU - Guerrier-Takada, Cecilia

AU - Altman, Sidney

PY - 2001

Y1 - 2001

N2 - A yeast three-hybrid system was employed to analyze interactions in vivo between H1 RNA, the RNA subunit of human nuclear RNase P, and eight of the protein subunits of the enzyme. The genetic analysis indicates that subunits Rpp21, Rpp29, Rpp30, and Rpp38 interact directly with H1 RNA. The results of direct UV crosslinking studies of the purified RNase P holoenzyme confirm the results of the three-hybrid assay.

AB - A yeast three-hybrid system was employed to analyze interactions in vivo between H1 RNA, the RNA subunit of human nuclear RNase P, and eight of the protein subunits of the enzyme. The genetic analysis indicates that subunits Rpp21, Rpp29, Rpp30, and Rpp38 interact directly with H1 RNA. The results of direct UV crosslinking studies of the purified RNase P holoenzyme confirm the results of the three-hybrid assay.

KW - HeLa cells

KW - Precursor tRNAs

KW - Three-hybrid assay

KW - UV crosslinking

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U2 - 10.1017/S1355838201010299

DO - 10.1017/S1355838201010299

M3 - Article

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AN - SCOPUS:0034960193

SN - 1355-8382

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SP - 937

EP - 941

JO - RNA

JF - RNA

IS - 7

ER -

Protein-RNA interactions in the subunits of human nuclear RNase P

Abstract

Keywords

ASJC Scopus subject areas

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