Protein-RNA interactions in the subunits of human nuclear RNase P

Taijiao Jiang, Cecilia Guerrier-Takada, Sidney Altman

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

A yeast three-hybrid system was employed to analyze interactions in vivo between H1 RNA, the RNA subunit of human nuclear RNase P, and eight of the protein subunits of the enzyme. The genetic analysis indicates that subunits Rpp21, Rpp29, Rpp30, and Rpp38 interact directly with H1 RNA. The results of direct UV crosslinking studies of the purified RNase P holoenzyme confirm the results of the three-hybrid assay.

Original languageEnglish (US)
Pages (from-to)937-941
Number of pages5
JournalRNA
Volume7
Issue number7
DOIs
StatePublished - Jul 16 2001
Externally publishedYes

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Keywords

  • HeLa cells
  • Precursor tRNAs
  • Three-hybrid assay
  • UV crosslinking

ASJC Scopus subject areas

  • Molecular Biology

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