Abstract
The genes for the protein (C5 protein) and RNA (M1 RNA) subunits of Escherichia coli RNase P have been subcloned and their products prepared in milligram quantities by rapid procedures. The interactions between the two subunits of the enzyme have been studied in vitro by a filter-binding technique. The stoichiometry of the subunits in the holoenzyme is 1:1. The dissociation constant for the specific interactions of the subunits in the holoenzyme complex is ~4 × 10-10 m. C5 protein also interacts with various RNA molecules in a non-specific manner with a dissociation constant of 2 × 10-8 to 6 × 10-8 m. Regions of M1 RNA required for interaction with C5 protein have been defined by deletion analysis and footprinting techniques. These interactions are localized primarily between nucleotides 82 to 96 and 170 to 270 of M1 RNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 835-848 |
| Number of pages | 14 |
| Journal | Journal of Molecular Biology |
| Volume | 202 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 20 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
Cite this
Protein-RNA interactions in the RNase P holoenzyme from Escherichia coli. / Vioque, Agustín; Arnez, John; Altman, Sidney.
In: Journal of Molecular Biology, Vol. 202, No. 4, 20.08.1988, p. 835-848.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protein-RNA interactions in the RNase P holoenzyme from Escherichia coli
AU - Vioque, Agustín
AU - Arnez, John
AU - Altman, Sidney
PY - 1988/8/20
Y1 - 1988/8/20
N2 - The genes for the protein (C5 protein) and RNA (M1 RNA) subunits of Escherichia coli RNase P have been subcloned and their products prepared in milligram quantities by rapid procedures. The interactions between the two subunits of the enzyme have been studied in vitro by a filter-binding technique. The stoichiometry of the subunits in the holoenzyme is 1:1. The dissociation constant for the specific interactions of the subunits in the holoenzyme complex is ~4 × 10-10 m. C5 protein also interacts with various RNA molecules in a non-specific manner with a dissociation constant of 2 × 10-8 to 6 × 10-8 m. Regions of M1 RNA required for interaction with C5 protein have been defined by deletion analysis and footprinting techniques. These interactions are localized primarily between nucleotides 82 to 96 and 170 to 270 of M1 RNA.
AB - The genes for the protein (C5 protein) and RNA (M1 RNA) subunits of Escherichia coli RNase P have been subcloned and their products prepared in milligram quantities by rapid procedures. The interactions between the two subunits of the enzyme have been studied in vitro by a filter-binding technique. The stoichiometry of the subunits in the holoenzyme is 1:1. The dissociation constant for the specific interactions of the subunits in the holoenzyme complex is ~4 × 10-10 m. C5 protein also interacts with various RNA molecules in a non-specific manner with a dissociation constant of 2 × 10-8 to 6 × 10-8 m. Regions of M1 RNA required for interaction with C5 protein have been defined by deletion analysis and footprinting techniques. These interactions are localized primarily between nucleotides 82 to 96 and 170 to 270 of M1 RNA.
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U2 - 10.1016/0022-2836(88)90562-1
DO - 10.1016/0022-2836(88)90562-1
M3 - Article
VL - 202
SP - 835
EP - 848
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 4
ER -