Protein identification and quantification for single cell analysis by coupling a microfluidic platform with MALDI-TOF

M. Yang, T. C. Chao, R. Nelson, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we present our progress towards protein identification and quantification with single cell sensitivity using the hyphenation of a microfluidic platform and MALDI mass spectrometry. In our previous approach, we estimated a sensitivity in the range of high abundant proteins in single cells (∼106 protein molecules) [1]. Here, we extend these preliminary studies to achieve a true quantitative approach via identification and isotope tagging of the apoptosis marker Bcl-2.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1039-1041
Number of pages3
Volume2
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

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Keywords

  • ITRAQ tags
  • MALDI-TOF
  • Microfluidic
  • Single cell analysis

ASJC Scopus subject areas

  • Bioengineering

Cite this

Yang, M., Chao, T. C., Nelson, R., & Ros, A. (2013). Protein identification and quantification for single cell analysis by coupling a microfluidic platform with MALDI-TOF. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 2, pp. 1039-1041). Chemical and Biological Microsystems Society.