Protein identification and quantification for single cell analysis by coupling a microfluidic platform with MALDI-TOF

M. Yang, T. C. Chao, R. Nelson, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we present our progress towards protein identification and quantification with single cell sensitivity using the hyphenation of a microfluidic platform and MALDI mass spectrometry. In our previous approach, we estimated a sensitivity in the range of high abundant proteins in single cells (∼106 protein molecules) [1]. Here, we extend these preliminary studies to achieve a true quantitative approach via identification and isotope tagging of the apoptosis marker Bcl-2.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1039-1041
Number of pages3
ISBN (Print)9781632666246
StatePublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • ITRAQ tags
  • MALDI-TOF
  • Microfluidic
  • Single cell analysis

ASJC Scopus subject areas

  • Bioengineering

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