Protein-facilitated gold nanoparticle formation as indicators of ionizing radiation

Amar Thaker, Karthik Pushpavanam, Tomasz Bista, Stephen Sapareto, Kaushal Rege, Brent L. Nannenga

Research output: Contribution to journalArticle

Abstract

The use of X-ray radiation in radiotherapy is a common treatment for many cancers. Despite several scientific advances, determination of radiation delivered to the patient remains a challenge due to the inherent limitations of existing dosimeters including fabrication and operation. Here, we describe a colorimetric nanosensor that exhibits unique changes in color as a function of therapeutically relevant radiation dose (3–15 Gy). The nanosensor is formulated using a gold salt and maltose-binding protein as a templating agent, which upon exposure to ionizing radiation is converted to gold nanoparticles. The formation of gold nanoparticles from colorless precursor salts renders a change in color that can be observed visually. The dose-dependent multicolored response was quantified through a simple ultraviolet–visible spectrophotometer and the peak shift associated with the different colored dispersions was used as a quantitative indicator of therapeutically relevant radiation doses. The ease of fabrication, visual color changes upon exposure to ionizing radiation, and quantitative read-out demonstrates the potential of protein-facilitated biomineralization approaches to promote the development of next-generation detectors for ionizing radiation.

Original languageEnglish (US)
Pages (from-to)3160-3167
Number of pages8
JournalBiotechnology and bioengineering
Volume116
Issue number12
DOIs
StatePublished - Dec 1 2019
Externally publishedYes

Fingerprint

Ionizing radiation
Ionizing Radiation
Gold
Nanoparticles
Nanosensors
Color
Radiation
Proteins
Dosimetry
Salts
X-Rays
Maltose-Binding Proteins
Biomineralization
Maltose
Fabrication
Dosimeters
Spectrophotometers
Radiotherapy
Dispersions
Detectors

Keywords

  • biomineralization
  • gold nanoparticles
  • nanobiotechnology
  • radiation dosimetry
  • sensors

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Protein-facilitated gold nanoparticle formation as indicators of ionizing radiation. / Thaker, Amar; Pushpavanam, Karthik; Bista, Tomasz; Sapareto, Stephen; Rege, Kaushal; Nannenga, Brent L.

In: Biotechnology and bioengineering, Vol. 116, No. 12, 01.12.2019, p. 3160-3167.

Research output: Contribution to journalArticle

Thaker, Amar ; Pushpavanam, Karthik ; Bista, Tomasz ; Sapareto, Stephen ; Rege, Kaushal ; Nannenga, Brent L. / Protein-facilitated gold nanoparticle formation as indicators of ionizing radiation. In: Biotechnology and bioengineering. 2019 ; Vol. 116, No. 12. pp. 3160-3167.
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