Protein expression during heat stress in thermo-intolerant and thermo-tolerant diatoms

Jeffrey M. Rousch, Scott E. Bingham, Milton R. Sommerfeld

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Diatoms (Chrysophyta) are photosynthetic microorganisms that are abundant in the natural environment and often associated with specific habitat and water quality conditions. Their significance as bioindicators and as exploitable sources of fine chemicals makes them desirable candidates for the study of stress responses. The protein expression of a thermo-intolerant (Phaeodactylum tricornutum) and thermo-tolerant (Chaetoceros muelleri) diatom following exposure to elevated temperature was investigated using one- and two-dimensional gel electrophoresis and Western blot analysis. It was determined using SDS PAGE with 35S-methionine labeled proteins and Western blot analysis using pea HSP70 antisera that higher temperatures and longer duration treatment were required to cause a noticeable stress response in C. muelleri compared to P. tricornutum. This may be explained by C. muelleri possessing higher amounts of constitutively expressed heat shock proteins, which allows these cells to rapidly adjust to temperature increases. Two-dimensional gel electrophoresis revealed that putative small heat shock proteins (smHSPs) may appear to play a role during heat stress in both diatoms, which is similar to the response in plants. SDS PAGE data are also presented characterizing the recovery of P. tricornutum after heat shock. These results suggest that there is a lag period between heat shock and stress protein synthesis in these thermo-intolerant cells. This supports the hypothesis that cells without higher amounts of constitutively expressed stress proteins have a greater sensitivity to increased temperature. Work is underway to identify particular stress proteins responsible for conveying thermo-tolerance and to determine if overexpression of these genes in thermo-intolerant diatoms affects their temperature sensitivity.

Original languageEnglish (US)
Pages (from-to)231-243
Number of pages13
JournalJournal of Experimental Marine Biology and Ecology
Volume306
Issue number2
DOIs
StatePublished - Aug 5 2004

Fingerprint

Bacillariophyceae
heat stress
Chaetoceros muelleri
diatom
protein synthesis
Phaeodactylum tricornutum
heat shock
protein
two-dimensional gel electrophoresis
temperature
heat shock proteins
polyacrylamide gel electrophoresis
stress response
Western blotting
Chrysophyceae
gene overexpression
electrokinesis
proteins
gel
cells

Keywords

  • Chaetoceros
  • Heat stress
  • Marine diatom
  • Phaeodactylum
  • Protein

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Protein expression during heat stress in thermo-intolerant and thermo-tolerant diatoms. / Rousch, Jeffrey M.; Bingham, Scott E.; Sommerfeld, Milton R.

In: Journal of Experimental Marine Biology and Ecology, Vol. 306, No. 2, 05.08.2004, p. 231-243.

Research output: Contribution to journalArticle

Rousch, Jeffrey M. ; Bingham, Scott E. ; Sommerfeld, Milton R. / Protein expression during heat stress in thermo-intolerant and thermo-tolerant diatoms. In: Journal of Experimental Marine Biology and Ecology. 2004 ; Vol. 306, No. 2. pp. 231-243.
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