Abstract
Aspergillus glucoamylase catalyzes hydrolysis of D-glucose from non-reducing ends of starch with an É300-fold (k^J Km) preference for the a-1, 4- over the a-l, 6-glucosidic linkage determined using the substrates maltose and iso-maltose. It is postulated that as most amylolytic enzymes act on either the a-1, 4- or a-l, 6-linkages, sequence comparison between active-site regions should enable the correlation of the substrate bond specificity with particular residues at key positions. Therefore, the already high bond-type selectivity in Aspergillus glucoamylase could theoretically be augmented further by three single mutations, Serll9 Tyr, Glyl83 Lys and Serl84 His, in two separate active-site regions. These mutants all had slight increases in activity as compared with the wild-type enzyme towards the a-l, 4-linked maltose; this was due to lower Km values as well as small decreases in activity towards isomaltose. This latter decrease in activity was a result of higher Km values and a decrease in fc^, for the Serl84â’ His mutant As a consequence, the selectivity of the three glucoamylase mutants for a-1, 4- over a-l, 6-linked disaccharides is enhanced 2.3- to 3.5-fold. In addition, the introduction of a cationic side chain in Glyl83â舤 ’ Lys and Serl84 â舤’ His glucoamylase, broadens the optimal pH range for activity towards acidic as well as alkaline conditions.
Original language | English (US) |
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Pages (from-to) | 1479-1484 |
Number of pages | 6 |
Journal | Protein Engineering, Design and Selection |
Volume | 7 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1994 |
Externally published | Yes |
Keywords
- Active-site mutants
- Active-site sequence com-parison
- Glucoamylase
- Specificity engineering
- Starch hydrolases
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Molecular Biology