Protein engineering of the relative specificity of glucoamylase from aspergillus awamori based on sequence similarities between starchdegrading enzymes

Michael R. Sierks, Michael R. Sierks

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Aspergillus glucoamylase catalyzes hydrolysis of D-glucose from non-reducing ends of starch with an É300-fold (k^J Km) preference for the a-1, 4- over the a-l, 6-glucosidic linkage determined using the substrates maltose and iso-maltose. It is postulated that as most amylolytic enzymes act on either the a-1, 4- or a-l, 6-linkages, sequence comparison between active-site regions should enable the correlation of the substrate bond specificity with particular residues at key positions. Therefore, the already high bond-type selectivity in Aspergillus glucoamylase could theoretically be augmented further by three single mutations, Serll9 Tyr, Glyl83 Lys and Serl84 His, in two separate active-site regions. These mutants all had slight increases in activity as compared with the wild-type enzyme towards the a-l, 4-linked maltose; this was due to lower Km values as well as small decreases in activity towards isomaltose. This latter decrease in activity was a result of higher Km values and a decrease in fc^, for the Serl84â’ His mutant As a consequence, the selectivity of the three glucoamylase mutants for a-1, 4- over a-l, 6-linked disaccharides is enhanced 2.3- to 3.5-fold. In addition, the introduction of a cationic side chain in Glyl83â舤 ’ Lys and Serl84 â舤’ His glucoamylase, broadens the optimal pH range for activity towards acidic as well as alkaline conditions.

Original languageEnglish (US)
Pages (from-to)1479-1484
Number of pages6
JournalProtein Engineering, Design and Selection
Volume7
Issue number12
DOIs
StatePublished - Dec 1994
Externally publishedYes

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Keywords

  • Active-site mutants
  • Active-site sequence com-parison
  • Glucoamylase
  • Specificity engineering
  • Starch hydrolases

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Molecular Biology

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