Protein dielectrophoresis: Advances, challenges, and applications

Asuka Nakano, Alexandra Ros

Research output: Contribution to journalReview article

83 Scopus citations

Abstract

Protein dielectrophoresis (DEP) has the potential to play an important role as a manipulation, fractionation, preconcentration, and separation method in bioanalysis and as manipulation tool for nanotechnological applications. The first demonstrations of protein DEP have been reported almost 20 years ago. Since then various experimental realizations to manipulate proteins by DEP as well as more targeted applications employing protein DEP have been demonstrated. This review summarizes the experimental studies in the field of protein DEP trapping and focusing as well as specific applications in separation, molecular patterning, on bioprobes and biosensors. While a comprehensive theoretical model describing protein DEP is still lacking we also attempt to provide an overview of the factors influencing protein DEP and relate to currently available theoretical models. We further point out the variations in experimental conditions used in the past to study the somewhat 20 proteins as well as the implications of protein molecular structure to the DEP response.

Original languageEnglish (US)
Pages (from-to)1085-1096
Number of pages12
JournalElectrophoresis
Volume34
Issue number7
DOIs
StatePublished - Apr 1 2013

Keywords

  • Applications
  • Dielectrophoresis
  • Focusing
  • Protein
  • Trapping

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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