TY - GEN
T1 - Protein assay in drinking water and reclaimed water
AU - Lee, Wontae
AU - Westerhoff, Paul
PY - 2007/12/1
Y1 - 2007/12/1
N2 - The Lowry and Bradford assays are appropriate for untreated or poorly treated wastewaters, but are not sensitive enough for low protein concentrations such as found in reclaimed waters or drinking waters. A biomedical assay, NanoOrange® (Molecular Probes Inc., OR) which has been used to measure protein content in cells, is very sensitive, but exhibited interferences from salts in water, especially from divalent ions. As such it is of limited use for bulk drinking water samples, but could be used on samples after dialysis pretreatment. For natural organic matter (NOM) isolates and cloud samples, the NanoOrange® method could be used without concern for salts. The colloidal fraction of NOM exhibited the highest protein content based upon the NanoOrange® reagent.
AB - The Lowry and Bradford assays are appropriate for untreated or poorly treated wastewaters, but are not sensitive enough for low protein concentrations such as found in reclaimed waters or drinking waters. A biomedical assay, NanoOrange® (Molecular Probes Inc., OR) which has been used to measure protein content in cells, is very sensitive, but exhibited interferences from salts in water, especially from divalent ions. As such it is of limited use for bulk drinking water samples, but could be used on samples after dialysis pretreatment. For natural organic matter (NOM) isolates and cloud samples, the NanoOrange® method could be used without concern for salts. The colloidal fraction of NOM exhibited the highest protein content based upon the NanoOrange® reagent.
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M3 - Conference contribution
AN - SCOPUS:84871560161
SN - 9781605600499
T3 - American Water Works Association - Water Quality Technology Conference and Exposition 2007: Fast Tracks to Water Quality
SP - 663
EP - 666
BT - American Water Works Association - Water Quality Technology Conference and Exposition 2007
T2 - Water Quality Technology Conference and Exposition 2007: Fast Tracks to Water Quality
Y2 - 4 November 2007 through 8 November 2007
ER -