TY - GEN
T1 - PROTEIN ANALYSIS FROM SMALL CELL ENSEMBLES BY AN INTEGRATED MICROFLUIDIC AND MASS SPECTROMETRY ASSAY
AU - Villarreal, Jorvani Cruz
AU - Kruithoff, Rory
AU - Ros, Robert
AU - Ros, Alexandra
N1 - Funding Information:
We thank NIH for supporting this project (Grant R21AG067488), Dr. Mouneimne (UofA Cancer Center) for sharing MCF-7 cells and Dr. Nikkhah (SBHSE, ASU) for the cell medium. We acknowledge the use of ASU MS Facility.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Understanding cell heterogeneity and protein expression levels at the single cell level is of high clinical and biological interest, but is rarely achieved with current technologies. Here, we show the detection and quantification of the apoptotic marker protein Bcl-2 from MCF-7 breast cancer cells with an integrated microfluidic device combined with MALDI mass spectrometry. We demonstrated effective lysis of cells on-chip and the capability of quantifying down to 2.63 x 1011 molecules of proteins from cells as an important milestone to further develop this approach into a protein single cell analysis platform.
AB - Understanding cell heterogeneity and protein expression levels at the single cell level is of high clinical and biological interest, but is rarely achieved with current technologies. Here, we show the detection and quantification of the apoptotic marker protein Bcl-2 from MCF-7 breast cancer cells with an integrated microfluidic device combined with MALDI mass spectrometry. We demonstrated effective lysis of cells on-chip and the capability of quantifying down to 2.63 x 1011 molecules of proteins from cells as an important milestone to further develop this approach into a protein single cell analysis platform.
KW - Cell lysis
KW - Mass spectrometry
KW - Microfluidics
KW - Protein quantification
UR - http://www.scopus.com/inward/record.url?scp=85136915780&partnerID=8YFLogxK
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M3 - Conference contribution
AN - SCOPUS:85136915780
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 831
EP - 832
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -