Protection from chemical modification of nucleotides in complexes of M1 RNA, the catalytic subunit of RNase P from E coli, and tRNA precursors

A. K. Knap, D. Wesolowski, S. Altman

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Certain nucleotides in M1 RNA, the catalytic RNA subunit of RNase P from E coli, are protected from chemical modification when M1 RNA forms complexes with tRNA precursor molecules (ES complexes). Many of these nucleotides are important in the formation of the Michaelis complex. In the presence of tRNA precursor molecules, the pattern of protection from chemical modification of a region in M1 RNA that resembles the E site in 23S rRNA is similar to the pattern of protection of the E site in the presence of deacylated tRNA. In the complex with the RNA enzyme, more nucleotides in the substrate become accessible to modification, an indication that the substrate is in an unfolded conformation under these conditions.

Original languageEnglish (US)
Pages (from-to)779-790
Number of pages12
JournalBiochimie
Volume72
Issue number11
DOIs
StatePublished - Nov 1990
Externally publishedYes

Keywords

  • M1 RNA
  • chemical modification
  • substrate binding
  • tRNA precursors

ASJC Scopus subject areas

  • Biochemistry

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