Protection conferred by recombinant Yersinia pestis antigens produced by a rapid and highly scalable plant expression system

Luca Santi, Anatoli Giritch, Chad J. Roy, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba, Robert Webb, Charles J. Arntzen, Hugh Mason

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

Plague is still an endemic disease in different regions of the world. Increasing reports of incidence, the discovery of antibiotic resistance strains, and concern about a potential use of the causative bacteria Yersinia pestis as an agent of biological warfare have highlighted the need for a safe, efficacious, and rapidly producible vaccine. The use of F1 and V antigens and the derived protein fusion F1-V has shown great potential as a protective vaccine in animal studies. Plants have been extensively studied for the production of pharmaceutical proteins as an inexpensive and scalable alternative to common expression systems. In the current study the recombinant plague antigens F1, V, and fusion protein F1-V were produced by transient expression in Nicotiana benthamiana by using a deconstructed tobacco mosaic virus-based system that allowed very rapid and extremely high levels of expression. All of the plant-derived purified antigens, administered s.c. to guinea pigs, generated systemic immune responses and provided protection against an aerosol challenge of virulent Y. pestis.

Original languageEnglish (US)
Pages (from-to)861-866
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number4
DOIs
StatePublished - Jan 24 2006

Keywords

  • Plague
  • Plant biotechnology
  • Recombinant subunit vaccine
  • Viral vector

ASJC Scopus subject areas

  • General

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