Protection by recombinant viral proteins against a respiratory challenge with virulent avian metapneumovirus

Parag Chary, M. Kariuki Njenga, Jagdev M. Sharma

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Protection by recombinant avian metapneumovirus (aMPV) N or M proteins against a respiratory challenge with virulent aMPV was examined. N, M or N + M proteins were administered intramuscularly (IM) with incomplete Freund's adjuvant (IFA) or by the oculonasal (ON) route with cholera toxin-B (CTB). Each turkey received 40 or 80 μg of each recombinant protein. Birds were considered protected against challenge if the challenge virus was not detectable in the choanal swabs by RT-PCR. At a dose of 40 μg/bird, N protein given with IFA by the IM route protected eight out of nine birds. M protein at the same dose protected three out of seven birds, while a combination of N + M proteins (40 μg each) protected three out of four birds. At a dose of 80 μg of each of N and M proteins per bird given with IFA by the IM route, 100% protection was achieved. ON immunization with a mixture of N and M proteins induced partial protection when the proteins were given with CTB; no detectable protection was noted without CTB. N and M proteins induced anti-aMPV antibodies, although protection against virulent virus challenge did not appear to be associated with the level or presence of antibodies.

Original languageEnglish (US)
Pages (from-to)427-432
Number of pages6
JournalVeterinary Immunology and Immunopathology
Volume108
Issue number3-4
DOIs
StatePublished - Dec 15 2005
Externally publishedYes

Fingerprint

Metapneumovirus
Avian metapneumovirus
viral proteins
Viral Proteins
Recombinant Proteins
recombinant proteins
Birds
Cholera Toxin
Avian Proteins
Proteins
cholera toxin
proteins
birds
adjuvants
Viruses
dosage
Antibodies
viruses
antibodies
Immunization

Keywords

  • Antibodies
  • Avian metapneumovirus
  • Immunization
  • Protection
  • Recombinant proteins
  • Turkeys

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Protection by recombinant viral proteins against a respiratory challenge with virulent avian metapneumovirus. / Chary, Parag; Njenga, M. Kariuki; Sharma, Jagdev M.

In: Veterinary Immunology and Immunopathology, Vol. 108, No. 3-4, 15.12.2005, p. 427-432.

Research output: Contribution to journalArticle

Chary, Parag ; Njenga, M. Kariuki ; Sharma, Jagdev M. / Protection by recombinant viral proteins against a respiratory challenge with virulent avian metapneumovirus. In: Veterinary Immunology and Immunopathology. 2005 ; Vol. 108, No. 3-4. pp. 427-432.
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