Protection Against Necrotic Enteritis in Broiler Chickens by Regulated Delayed Lysis Salmonella Vaccines

Yanlong Jiang, Hua Mo, Crystal Willingham, Shifeng Wang, Jie Yeun Park, Wei Kong, Kenneth L. Roland, Roy Curtiss

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Necrotic enteritis (NE), caused by Gram-positive Clostridium perfringens type A strains, has gained more attention in the broiler industry due to governmental restrictions affecting the use of growth-promoting antibiotics in feed. To date, there is only one commercial NE vaccine available, based on the C. perfringens alpha toxin. However, recent work has suggested that the NetB toxin, not alpha toxin, is the most critical virulence factor for causing NE. These findings notwithstanding, it is clear from prior research that immune responses against both toxins can provide some protection against NE. In this study, we delivered a carboxyl-terminal fragment of alpha toxin and a GST-NetB fusion protein using a novel attenuated Salmonella vaccine strain designed to lyse after 6-10 rounds of replication in the chicken host. We immunized birds with vaccine strains producing each protein individually, a mixture of the two strains, or with a single vaccine strain that produced both proteins. Immunization with strains producing either of the single proteins was not protective, but immunization with a mixture of the two or with a single strain producing both proteins resulted in protective immunity. The vaccine strain synthesizing both PlcC and GST-NetB was able to elicit strong production of intestinal IgA, IgY, and IgM antibodies and significantly protect broilers against C. perfringens challenge against both mild and severe challenges. Although not part of our experimental plan, the broiler chicks we obtained for these studies were apparently contaminated during transit from the hatchery with group D Salmonella. Despite this drawback, the vaccines worked well, indicating applicability to real-world conditions.

Original languageEnglish (US)
Pages (from-to)475-485
Number of pages11
JournalAvian Diseases
Volume59
Issue number4
DOIs
StatePublished - Dec 1 2015

Fingerprint

Salmonella Vaccines
necrotic enteritis
Enteritis
Chickens
Salmonella
Vaccines
broiler chickens
vaccines
toxins
Clostridium perfringens
Proteins
Immunization
Attenuated Vaccines
proteins
Virulence Factors
immunization
Immunoglobulin A
Birds
Immunoglobulin M
Clostridium perfringens A

Keywords

  • Clostridium perfringens
  • Salmonella
  • vaccine protection necrotic enteritis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Immunology and Microbiology(all)

Cite this

Protection Against Necrotic Enteritis in Broiler Chickens by Regulated Delayed Lysis Salmonella Vaccines. / Jiang, Yanlong; Mo, Hua; Willingham, Crystal; Wang, Shifeng; Park, Jie Yeun; Kong, Wei; Roland, Kenneth L.; Curtiss, Roy.

In: Avian Diseases, Vol. 59, No. 4, 01.12.2015, p. 475-485.

Research output: Contribution to journalArticle

Jiang, Y, Mo, H, Willingham, C, Wang, S, Park, JY, Kong, W, Roland, KL & Curtiss, R 2015, 'Protection Against Necrotic Enteritis in Broiler Chickens by Regulated Delayed Lysis Salmonella Vaccines', Avian Diseases, vol. 59, no. 4, pp. 475-485. https://doi.org/10.1637/11094-041715-Reg
Jiang, Yanlong ; Mo, Hua ; Willingham, Crystal ; Wang, Shifeng ; Park, Jie Yeun ; Kong, Wei ; Roland, Kenneth L. ; Curtiss, Roy. / Protection Against Necrotic Enteritis in Broiler Chickens by Regulated Delayed Lysis Salmonella Vaccines. In: Avian Diseases. 2015 ; Vol. 59, No. 4. pp. 475-485.
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