Protease probes built from DNA: Multispectral fluorescent DNA-peptide conjugates as caspase chemosensors

Nan Dai; Jia Guo; Yin Nah Teo; Eric T. Kool

doi:10.1002/anie.201007805

Protease probes built from DNA: Multispectral fluorescent DNA-peptide conjugates as caspase chemosensors

Nan Dai, Jia Guo, Yin Nah Teo, Eric T. Kool

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Shaken, not stirred: A novel design for protease sensors is described, in which peptides are conjugated to a DNA backbone carrying multiple fluorophores. The multispectral oligodeoxyfluoroside (ODF) fluorophores are strongly quenched by a dabcyl group at the end of each peptide. In vitro and cellular selectivity assays showed that a mixture of three sensors could be used to identify different caspase activities by the fluorescence outcome (see picture).

Original language	English (US)
Pages (from-to)	5105-5109
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	22
DOIs	https://doi.org/10.1002/anie.201007805
State	Published - May 23 2011
Externally published	Yes

Keywords

apoptosis
caspase
fluorescent probes
imaging agents
oligodeoxyfluoroside

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201007805

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abstract = "Shaken, not stirred: A novel design for protease sensors is described, in which peptides are conjugated to a DNA backbone carrying multiple fluorophores. The multispectral oligodeoxyfluoroside (ODF) fluorophores are strongly quenched by a dabcyl group at the end of each peptide. In vitro and cellular selectivity assays showed that a mixture of three sensors could be used to identify different caspase activities by the fluorescence outcome (see picture).",

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AU - Dai, Nan

AU - Guo, Jia

AU - Teo, Yin Nah

AU - Kool, Eric T.

PY - 2011/5/23

Y1 - 2011/5/23

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KW - apoptosis

KW - caspase

KW - fluorescent probes

KW - imaging agents

KW - oligodeoxyfluoroside

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Protease probes built from DNA: Multispectral fluorescent DNA-peptide conjugates as caspase chemosensors

Abstract

Keywords

ASJC Scopus subject areas

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