Protease probes built from DNA: Multispectral fluorescent DNA-peptide conjugates as caspase chemosensors

Nan Dai, Jia Guo, Yin Nah Teo, Eric T. Kool

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Shaken, not stirred: A novel design for protease sensors is described, in which peptides are conjugated to a DNA backbone carrying multiple fluorophores. The multispectral oligodeoxyfluoroside (ODF) fluorophores are strongly quenched by a dabcyl group at the end of each peptide. In vitro and cellular selectivity assays showed that a mixture of three sensors could be used to identify different caspase activities by the fluorescence outcome (see picture).

Original languageEnglish (US)
Pages (from-to)5105-5109
Number of pages5
JournalAngewandte Chemie - International Edition
Volume50
Issue number22
DOIs
StatePublished - May 23 2011
Externally publishedYes

Fingerprint

Fluorophores
DNA Probes
Caspases
Peptides
DNA
Peptide Hydrolases
Sensors
Assays
Fluorescence

Keywords

  • apoptosis
  • caspase
  • fluorescent probes
  • imaging agents
  • oligodeoxyfluoroside

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Protease probes built from DNA : Multispectral fluorescent DNA-peptide conjugates as caspase chemosensors. / Dai, Nan; Guo, Jia; Teo, Yin Nah; Kool, Eric T.

In: Angewandte Chemie - International Edition, Vol. 50, No. 22, 23.05.2011, p. 5105-5109.

Research output: Contribution to journalArticle

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