Protease probes built from DNA: Multispectral fluorescent DNA-peptide conjugates as caspase chemosensors

Nan Dai, Jia Guo, Yin Nah Teo, Eric T. Kool

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Shaken, not stirred: A novel design for protease sensors is described, in which peptides are conjugated to a DNA backbone carrying multiple fluorophores. The multispectral oligodeoxyfluoroside (ODF) fluorophores are strongly quenched by a dabcyl group at the end of each peptide. In vitro and cellular selectivity assays showed that a mixture of three sensors could be used to identify different caspase activities by the fluorescence outcome (see picture).

Original languageEnglish (US)
Pages (from-to)5105-5109
Number of pages5
JournalAngewandte Chemie - International Edition
Volume50
Issue number22
DOIs
StatePublished - May 23 2011
Externally publishedYes

Keywords

  • apoptosis
  • caspase
  • fluorescent probes
  • imaging agents
  • oligodeoxyfluoroside

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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