Protease-deficient DegP suppresses lethal effects of a mutant OmpC protein by its capture

Maria CastilloKeller, Rajeev Misra

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The expression of assembly-defective outer membrane proteins can confer lethality if they are not degraded by envelope proteases. We report here that the expression of a mutant OmpC protein, OmpC2cys which forms disulfide bonds in the periplasm due to the presence of two non-native cysteine residues, is lethal in cells lacking the major periplasmic protease, DegP. This lethality is not observed in dsbA strains that have diminished ability to form periplasmic disulfide bonds. Our data show that this OmpC2Cys mediated lethality in a degP:: Kmr dsbA+ background can be reversed by a DegP variant, DegPS210A, that is devoid of its proteolytic activity but retains its reported chaperone activity. However, DegPS210A does not reverse the lethal effect of OmpC2Cys, by correcting its assembly but rather by capturing misfolded mutant OmpC polypeptides and thus removing them from the assembly pathway. Displacement of OmpC2Cys by DegPS210A also alleviates the negative effect that the mutant OmpC protein has on wild-type OmpF.

Original languageEnglish (US)
Pages (from-to)148-154
Number of pages7
JournalJournal of Bacteriology
Volume185
Issue number1
DOIs
StatePublished - Jan 2003

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Mutant Proteins
Disulfides
Periplasm
Cysteine
Membrane Proteins
Peptide Hydrolases
Peptides
DegP protease
OmpC protein

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Protease-deficient DegP suppresses lethal effects of a mutant OmpC protein by its capture. / CastilloKeller, Maria; Misra, Rajeev.

In: Journal of Bacteriology, Vol. 185, No. 1, 01.2003, p. 148-154.

Research output: Contribution to journalArticle

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