Prostaglandin E2 Increases Growth and Motility of Colorectal Carcinoma Cells

Hongmiao Sheng, Jinyi Shao, M. Kay Washington, Raymond N. DuBois

Research output: Contribution to journalArticle

552 Scopus citations

Abstract

Chronic use of nonsteroidal anti-inflammatory drugs results in a significant reduction of risk and mortality from colorectal cancer in humans. All of the mechanism(s) by which nonsteroidal anti-inflammatory drugs exert their protective effects are not completely understood, but they are known to inhibit cyclooxygenase activity. The cyclooxygenase enzymes catalyze a key reaction in the conversion of arachidonic acid to prostaglandins, such as prostaglandin E2 (PGE2). Here we demonstrate that PGE 2 treatment of LS-174 human colorectal carcinoma cells leads to increased motility and changes in cell shape. The prostaglandin EP4 receptor signaling pathway appears to play a role in transducing signals which regulate these effects. PGE2 treatment results in an activation of phosphatidylinositol 3-kinase/protein kinase B pathway that is required for the PGE2-induced changes in carcinoma cell motility and colony morphology. Our results suggest that PGE2 might enhance the invasive potential of colorectal carcinoma cells via activation of major intracellular signal transduction pathways not previously reported to be regulated by prostaglandins.

Original languageEnglish (US)
Pages (from-to)18075-18081
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number21
DOIs
Publication statusPublished - Jan 25 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

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