Propagation and behavior in chicken kidney cultures of the agent associated with classical marek's disease

J. M. Sharma, S. G. Kenzy, A. Rissberger

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A classical isolate (WSU-GF) of Marek's disease herpesvirus (MDHV), isolated from the kidneys of a chicken with clinical symptoms of the disease, was successfully propagated in chicken kidney (CK) cultures. The cytopathic effect (CPE) was similar to that produced by the herpesviruses of group B and could be inhibited by 5-iododeoxyuridine. With continued serial passage of MDHV in CK cultures, the character of CPE was changed in that the polykaryocytes became much larger and the type-A inclusions more frequent. Based on the specific CPE, a tissue culture assay system for MDHV was standardized. It was found that a cell was the unit of infection. A pH of 7.5 and an incubation temperature of 37°C in CO2 atmosphere were optimal for the expression of CPE by MDHV. A minimum contact of 12 hours was necessary between the infected and the normal CK cells to obtain a maximum number of foci of infection. It appeared that the cells of infected cultures became infectious before attaining a rounded, refractile state.

Original languageEnglish (US)
Pages (from-to)907-916
Number of pages10
JournalJournal of the National Cancer Institute
Volume43
Issue number4
DOIs
StatePublished - Oct 1969

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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