Programmed biomolecule delivery to enable and direct cell migration for connective tissue repair

Feini Qu, Julianne Holloway, John L. Esterhai, Jason A. Burdick, Robert L. Mauck

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Dense connective tissue injuries have limited repair, due to the paucity of cells at the wound site. We hypothesize that decreasing the density of the local extracellular matrix (ECM) in conjunction with releasing chemoattractive signals increases cellularity and tissue formation after injury. Using the knee meniscus as a model system, we query interstitial cell migration in the context of migratory barriers using a novel tissue Boyden chamber and show that a gradient of platelet-derived growth factor-AB (PDGF-AB) expedites migration through native tissue. To implement these signals in situ, we develop nanofibrous scaffolds with distinct fiber fractions that sequentially release active collagenase (to increase ECM porosity) and PDGF-AB (to attract endogenous cells) in a localized and coordinated manner. We show that, when placed into a meniscal defect, the controlled release of collagenase and PDGF-AB increases cellularity at the interface and within the scaffold, as well as integration with the surrounding tissue.

Original languageEnglish (US)
Article number1780
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

Fingerprint

connective tissue
Biomolecules
Connective Tissue
Cell Movement
delivery
Repair
platelets
Tissue
Collagenases
cells
Extracellular Matrix
Wounds and Injuries
Scaffolds
Porosity
releasing
matrices
Knee
interstitials
chambers
porosity

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Programmed biomolecule delivery to enable and direct cell migration for connective tissue repair. / Qu, Feini; Holloway, Julianne; Esterhai, John L.; Burdick, Jason A.; Mauck, Robert L.

In: Nature Communications, Vol. 8, No. 1, 1780, 01.12.2017.

Research output: Contribution to journalArticle

Qu, Feini ; Holloway, Julianne ; Esterhai, John L. ; Burdick, Jason A. ; Mauck, Robert L. / Programmed biomolecule delivery to enable and direct cell migration for connective tissue repair. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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