Production of rotavirus-like particles in tomato (Lycopersicon esculentum L.) fruit by expression of capsid proteins VP2 and VP6 and immunological studies

Sergio Saldaña, Fernando Esquivel Guadarrama, Teresa De Jesús Olivera Flores, Nancy Arias, Susana López, Carlos Arias, Roberto Ruiz-Medrano, Hugh Mason, Tsafrir Leket-Mor, Liz Richter, Charles J. Arntzen, Miguel A. Gómez Lim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A number of different antigens have been successfully expressed in transgenic plants, and some are currently being evaluated as orally delivered vaccines. Here we report the successful expression of rotavirus capsid proteins VP2 and VP6 in fruits of transgenic tomato plants. By western blot analysis, using specific antibodies, we determined that the VP2 and VP6 produced in plants have molecular weights similar to those found in native rotavirus. The plant-synthesized VP6 protein retained the capacity to form trimers. We were able to recover rotavirus virus-like particles from tomato fruit (i.e., tomatoes) by centrifugation on a sucrose cushion and to visualize them by electron microscopy. This result indicated that VP2/VP6 can self-assemble into virus-like particles (VLPs) in plant cells, even though only a small proportion of VP2/VP6 assembled into VLPs. To investigate immunogenicity, adult mice were immunized intraperitoneally (i.p.) three times with a protein extract from a transgenic tomatoes in adjuvant. We found that the transgenic tomato extract induced detectable levels of anti-rotavirus antibodies in serum; however, we did not determine the contribution of either the free rotavirus proteins or the VLPs to the induction of the antibody response. These results suggest the potential of plant-based rotavirus VLPs for the development of a vaccine against rotavirus infection.

Original languageEnglish (US)
Pages (from-to)42-53
Number of pages12
JournalViral Immunology
Volume19
Issue number1
DOIs
StatePublished - 2006

Fingerprint

Rotavirus
Capsid Proteins
Lycopersicon esculentum
Fruit
Virion
Genetically Modified Plants
Vaccines
Rotavirus Infections
Proteins
Plant Cells
Centrifugation
Antibody Formation
Sucrose
Anti-Idiotypic Antibodies
Electron Microscopy
Molecular Weight
Western Blotting
Antigens
Antibodies
Serum

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Saldaña, S., Guadarrama, F. E., Olivera Flores, T. D. J., Arias, N., López, S., Arias, C., ... Gómez Lim, M. A. (2006). Production of rotavirus-like particles in tomato (Lycopersicon esculentum L.) fruit by expression of capsid proteins VP2 and VP6 and immunological studies. Viral Immunology, 19(1), 42-53. https://doi.org/10.1089/vim.2006.19.42

Production of rotavirus-like particles in tomato (Lycopersicon esculentum L.) fruit by expression of capsid proteins VP2 and VP6 and immunological studies. / Saldaña, Sergio; Guadarrama, Fernando Esquivel; Olivera Flores, Teresa De Jesús; Arias, Nancy; López, Susana; Arias, Carlos; Ruiz-Medrano, Roberto; Mason, Hugh; Leket-Mor, Tsafrir; Richter, Liz; Arntzen, Charles J.; Gómez Lim, Miguel A.

In: Viral Immunology, Vol. 19, No. 1, 2006, p. 42-53.

Research output: Contribution to journalArticle

Saldaña, S, Guadarrama, FE, Olivera Flores, TDJ, Arias, N, López, S, Arias, C, Ruiz-Medrano, R, Mason, H, Leket-Mor, T, Richter, L, Arntzen, CJ & Gómez Lim, MA 2006, 'Production of rotavirus-like particles in tomato (Lycopersicon esculentum L.) fruit by expression of capsid proteins VP2 and VP6 and immunological studies', Viral Immunology, vol. 19, no. 1, pp. 42-53. https://doi.org/10.1089/vim.2006.19.42
Saldaña, Sergio ; Guadarrama, Fernando Esquivel ; Olivera Flores, Teresa De Jesús ; Arias, Nancy ; López, Susana ; Arias, Carlos ; Ruiz-Medrano, Roberto ; Mason, Hugh ; Leket-Mor, Tsafrir ; Richter, Liz ; Arntzen, Charles J. ; Gómez Lim, Miguel A. / Production of rotavirus-like particles in tomato (Lycopersicon esculentum L.) fruit by expression of capsid proteins VP2 and VP6 and immunological studies. In: Viral Immunology. 2006 ; Vol. 19, No. 1. pp. 42-53.
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