Production of myxoma virus gateway entry and expression libraries and validation of viral protein expression

Sherin E. Smallwood, Masmudur Rahman, Steven J. Werden, Maria Fernanda Martino, Douglas McFadden

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Invitrogen's Gateway technology is a recombination-based cloning method that allows for rapid transfer of numerous open reading frames (ORFs) into multiple plasmid vectors, making it useful for diverse high-throughput applications. Gateway technology has been utilized to create anORF library for Myxoma virus (MYXV), a member of the Poxviridae family of DNA viruses. MYXV is the prototype virus for the genus Leporipoxvirus, and is pathogenic only in European rabbits. MYXV replicates exclusively in the host cell cytoplasm, and its genome encodes 171 ORFs. A number of these ORFs encode proteins that interfere with or modulate host defense mechanisms, particularly the inflammatory responses. Furthermore, MYXV is able to productively infect a variety of human cancer cell lines and is being developed as an oncolytic virus for treating human cancers. MYXV is therefore an excellent model for studying poxvirus biology, pathogenesis, and host tropism, and a good candidate for ORFeome development.

Original languageEnglish (US)
JournalCurrent Protocols in Microbiology
Issue numberSUPPL.21
DOIs
StatePublished - Aug 29 2011
Externally publishedYes

Fingerprint

Myxoma virus
Virus Internalization
Viral Proteins
Open Reading Frames
Poxviridae
Leporipoxvirus
Viral Tropism
Oncolytic Viruses
Technology
DNA Viruses
Defense Mechanisms
Genetic Recombination
Libraries
Organism Cloning
Neoplasms
Cytoplasm
Plasmids
Genome
Rabbits
Viruses

Keywords

  • Entry clone
  • Expression clone
  • Gateway recombination cloning
  • Myxoma virus
  • Protein expression

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

Production of myxoma virus gateway entry and expression libraries and validation of viral protein expression. / Smallwood, Sherin E.; Rahman, Masmudur; Werden, Steven J.; Martino, Maria Fernanda; McFadden, Douglas.

In: Current Protocols in Microbiology, No. SUPPL.21, 29.08.2011.

Research output: Contribution to journalArticle

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