Abstract
The production, characterization, and antioxidant capacity of the carotenoid fucoxanthin from the marine diatom Odontella aurita were investigated. The results showed that low light and nitrogen-replete culture medium enhanced the biosynthesis of fucoxanthin. The maximum biomass concentration of 6.36 g L-1 and maximum fucoxanthin concentration of 18.47 mg g-1 were obtained in cultures grown in a bubble column photobioreactor (Ø 3.0 cm inner diameter), resulting in a fucoxanthin volumetric productivity of 7.96 mg L-1 day-1. A slight reduction in biomass production was observed in the scaling up of O. aurita culture in a flat plate photobioreactor, yet yielded a comparable fucoxanthin volumetric productivity. A rapid method was developed for extraction and purification of fucoxanthin. The purified fucoxanthin was identified as all-trans-fucoxanthin, which exhibited strong antioxidant properties, with the effective concentration for 50% scavenging (EC50) of 1,1-dihpenyl-2- picrylhydrazyl (DPPH) radical and 2,2′-Azino-bis(3-ethylbenzthiazoline-6- sulfonic acid (ABTS) radical being 0.14 and 0.03 mg mL-1, respectively. Our results suggested that O. aurita can be a natural source of fucoxanthin for human health and nutrition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2667-2681 |
| Number of pages | 15 |
| Journal | Marine Drugs |
| Volume | 11 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 2013 |
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Keywords
- Antioxidant
- Fucoxanthin
- Microalgae
- Odontella aurita
- Photobioreactor
ASJC Scopus subject areas
- Drug Discovery
Cite this
Production, characterization, and antioxidant activity of fucoxanthin from the marine diatom odontella aurita. / Xia, Song; Wang, Ke; Wan, Linglin; Li, Aifen; Hu, Qiang; Zhang, Chengwu.
In: Marine Drugs, Vol. 11, No. 7, 07.2013, p. 2667-2681.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production, characterization, and antioxidant activity of fucoxanthin from the marine diatom odontella aurita
AU - Xia, Song
AU - Wang, Ke
AU - Wan, Linglin
AU - Li, Aifen
AU - Hu, Qiang
AU - Zhang, Chengwu
PY - 2013/7
Y1 - 2013/7
N2 - The production, characterization, and antioxidant capacity of the carotenoid fucoxanthin from the marine diatom Odontella aurita were investigated. The results showed that low light and nitrogen-replete culture medium enhanced the biosynthesis of fucoxanthin. The maximum biomass concentration of 6.36 g L-1 and maximum fucoxanthin concentration of 18.47 mg g-1 were obtained in cultures grown in a bubble column photobioreactor (Ø 3.0 cm inner diameter), resulting in a fucoxanthin volumetric productivity of 7.96 mg L-1 day-1. A slight reduction in biomass production was observed in the scaling up of O. aurita culture in a flat plate photobioreactor, yet yielded a comparable fucoxanthin volumetric productivity. A rapid method was developed for extraction and purification of fucoxanthin. The purified fucoxanthin was identified as all-trans-fucoxanthin, which exhibited strong antioxidant properties, with the effective concentration for 50% scavenging (EC50) of 1,1-dihpenyl-2- picrylhydrazyl (DPPH) radical and 2,2′-Azino-bis(3-ethylbenzthiazoline-6- sulfonic acid (ABTS) radical being 0.14 and 0.03 mg mL-1, respectively. Our results suggested that O. aurita can be a natural source of fucoxanthin for human health and nutrition.
AB - The production, characterization, and antioxidant capacity of the carotenoid fucoxanthin from the marine diatom Odontella aurita were investigated. The results showed that low light and nitrogen-replete culture medium enhanced the biosynthesis of fucoxanthin. The maximum biomass concentration of 6.36 g L-1 and maximum fucoxanthin concentration of 18.47 mg g-1 were obtained in cultures grown in a bubble column photobioreactor (Ø 3.0 cm inner diameter), resulting in a fucoxanthin volumetric productivity of 7.96 mg L-1 day-1. A slight reduction in biomass production was observed in the scaling up of O. aurita culture in a flat plate photobioreactor, yet yielded a comparable fucoxanthin volumetric productivity. A rapid method was developed for extraction and purification of fucoxanthin. The purified fucoxanthin was identified as all-trans-fucoxanthin, which exhibited strong antioxidant properties, with the effective concentration for 50% scavenging (EC50) of 1,1-dihpenyl-2- picrylhydrazyl (DPPH) radical and 2,2′-Azino-bis(3-ethylbenzthiazoline-6- sulfonic acid (ABTS) radical being 0.14 and 0.03 mg mL-1, respectively. Our results suggested that O. aurita can be a natural source of fucoxanthin for human health and nutrition.
KW - Antioxidant
KW - Fucoxanthin
KW - Microalgae
KW - Odontella aurita
KW - Photobioreactor
UR - http://www.scopus.com/inward/record.url?scp=84880883616&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880883616&partnerID=8YFLogxK
U2 - 10.3390/md11072667
DO - 10.3390/md11072667
M3 - Article
VL - 11
SP - 2667
EP - 2681
JO - Marine Drugs
JF - Marine Drugs
SN - 1660-3397
IS - 7
ER -