Abstract

Thyroglobulin (Tg) is a sensitive indicator of persistent or recurrent differentiated thyroid cancer of follicular cell origin. Detection of Tg in human serum is challenging as bio-receptors, such as anti-Tg, used in immunoassay have relatively weak binding affinity. We engineer sensing surfaces using the competitive adsorption of proteins, termed the Vroman Effect. Coupled with Surface Plasmon Resonance, the "cross-responsive" interactions of Tg on the engineered surfaces produce uniquely distinguishable multiple signature patterns, which are discriminated using Linear Discriminant Analysis. Tg-spiked samples, down to 2 ng/ml Tg in undiluted human serum, are sensitively and selectively discriminated from the control (undiluted human serum).

Original languageEnglish (US)
Article number143703
JournalApplied Physics Letters
Volume105
Issue number14
DOIs
StatePublished - Oct 6 2014

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pattern recognition
serums
proteins
adsorption
immunoassay
surface plasmon resonance
engineers
affinity
cancer
signatures
cells
interactions

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Probing thyroglobulin in undiluted human serum based on pattern recognition and competitive adsorption of proteins. / Wang, Ran; Huang, Shuai; Li, Jing; Chae, Junseok.

In: Applied Physics Letters, Vol. 105, No. 14, 143703, 06.10.2014.

Research output: Contribution to journalArticle

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