Probing the low-frequency vibrational modes of viruses with Raman scattering - Bacteriophage M13 in water

Kong-Thon Tsen, Eric C. Dykeman, Otto F. Sankey, Shaw Wei D Tsen, Nien Tsung Lin, Juliann G. Kiang

Research output: Contribution to journalArticle

10 Scopus citations


Raman spectroscopy is used to study low-wave-number (≤ 20 cm -1) acoustic vibrations of the M13 phage. A well-defined Raman line is observed at around 8.5 cm-1. The experimental results are compared with theoretical calculations based on an elastic continuum model and appropriate Raman selection rules derived from a bond polarizability model. The observed Raman mode is shown to belong to one of the Raman-active axial modes of the M13 phage protein coat. It is expected that the detection and characterization of this low-frequency vibrational mode can be used for applications in biomedical nanotechnology such as for monitoring the process of virus functionalization and self-assembly.

Original languageEnglish (US)
Article number024009
JournalJournal of biomedical optics
Issue number2
StatePublished - Mar 1 2007



  • Laser applications
  • Laser spectroscopy
  • Lasers in medicine
  • Raman effect
  • Raman spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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