Probing Single Molecule Binding and Free Energy Profile with Plasmonic Imaging of Nanoparticles

Hui Wang, Zhuodong Tang, Yan Wang, Guangzhong Ma, Nongjian Tao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Measuring binding between molecules is critical for understanding basic biochemical processes, developing molecular diagnosis, and screening drugs. Here we study molecular binding at the single molecule level by attaching nanoparticles to the molecular binding pairs. We track the thermal fluctuations of the individual nanoparticles with sub-nanometer precision using a plasmonic scattering imaging technique and show that the fluctuations are controlled by the molecular binding pairs rather than by the nanoparticles. Analysis of the thermal fluctuations provides unique information on molecular binding, including binding energy profile, effective spring constant, and switching between single and multiple molecular binding events. The method provides new insights into molecular binding and also allows one to differentiate nonspecific binding from specific binding, which has been a difficult task in biosensors.

Original languageEnglish (US)
Pages (from-to)16071-16078
Number of pages8
JournalJournal of the American Chemical Society
Volume141
Issue number40
DOIs
StatePublished - Oct 9 2019
Externally publishedYes

Fingerprint

Binding energy
Nanoparticles
Free energy
Imaging techniques
Molecules
Biochemical Phenomena
Hot Temperature
Preclinical Drug Evaluations
Biosensing Techniques
Biosensors
Screening
Scattering
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Probing Single Molecule Binding and Free Energy Profile with Plasmonic Imaging of Nanoparticles. / Wang, Hui; Tang, Zhuodong; Wang, Yan; Ma, Guangzhong; Tao, Nongjian.

In: Journal of the American Chemical Society, Vol. 141, No. 40, 09.10.2019, p. 16071-16078.

Research output: Contribution to journalArticle

Wang, Hui ; Tang, Zhuodong ; Wang, Yan ; Ma, Guangzhong ; Tao, Nongjian. / Probing Single Molecule Binding and Free Energy Profile with Plasmonic Imaging of Nanoparticles. In: Journal of the American Chemical Society. 2019 ; Vol. 141, No. 40. pp. 16071-16078.
@article{83f23f2c81354f3ca762cfbc6cf43398,
title = "Probing Single Molecule Binding and Free Energy Profile with Plasmonic Imaging of Nanoparticles",
abstract = "Measuring binding between molecules is critical for understanding basic biochemical processes, developing molecular diagnosis, and screening drugs. Here we study molecular binding at the single molecule level by attaching nanoparticles to the molecular binding pairs. We track the thermal fluctuations of the individual nanoparticles with sub-nanometer precision using a plasmonic scattering imaging technique and show that the fluctuations are controlled by the molecular binding pairs rather than by the nanoparticles. Analysis of the thermal fluctuations provides unique information on molecular binding, including binding energy profile, effective spring constant, and switching between single and multiple molecular binding events. The method provides new insights into molecular binding and also allows one to differentiate nonspecific binding from specific binding, which has been a difficult task in biosensors.",
author = "Hui Wang and Zhuodong Tang and Yan Wang and Guangzhong Ma and Nongjian Tao",
year = "2019",
month = "10",
day = "9",
doi = "10.1021/jacs.9b08405",
language = "English (US)",
volume = "141",
pages = "16071--16078",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "40",

}

TY - JOUR

T1 - Probing Single Molecule Binding and Free Energy Profile with Plasmonic Imaging of Nanoparticles

AU - Wang, Hui

AU - Tang, Zhuodong

AU - Wang, Yan

AU - Ma, Guangzhong

AU - Tao, Nongjian

PY - 2019/10/9

Y1 - 2019/10/9

N2 - Measuring binding between molecules is critical for understanding basic biochemical processes, developing molecular diagnosis, and screening drugs. Here we study molecular binding at the single molecule level by attaching nanoparticles to the molecular binding pairs. We track the thermal fluctuations of the individual nanoparticles with sub-nanometer precision using a plasmonic scattering imaging technique and show that the fluctuations are controlled by the molecular binding pairs rather than by the nanoparticles. Analysis of the thermal fluctuations provides unique information on molecular binding, including binding energy profile, effective spring constant, and switching between single and multiple molecular binding events. The method provides new insights into molecular binding and also allows one to differentiate nonspecific binding from specific binding, which has been a difficult task in biosensors.

AB - Measuring binding between molecules is critical for understanding basic biochemical processes, developing molecular diagnosis, and screening drugs. Here we study molecular binding at the single molecule level by attaching nanoparticles to the molecular binding pairs. We track the thermal fluctuations of the individual nanoparticles with sub-nanometer precision using a plasmonic scattering imaging technique and show that the fluctuations are controlled by the molecular binding pairs rather than by the nanoparticles. Analysis of the thermal fluctuations provides unique information on molecular binding, including binding energy profile, effective spring constant, and switching between single and multiple molecular binding events. The method provides new insights into molecular binding and also allows one to differentiate nonspecific binding from specific binding, which has been a difficult task in biosensors.

UR - http://www.scopus.com/inward/record.url?scp=85072961302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072961302&partnerID=8YFLogxK

U2 - 10.1021/jacs.9b08405

DO - 10.1021/jacs.9b08405

M3 - Article

C2 - 31525042

AN - SCOPUS:85072961302

VL - 141

SP - 16071

EP - 16078

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 40

ER -