Probing protein nanoassemblies in neurodegenerative disease

Min S. Wang, Michael Sierks

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Atomic force microscopy (AFM) has been utilized in many biological applications and has evolved into a useful tool for structural characterization and for direct measurement of intermolecular forces at the nanoscale level. AFM offers unique advantages compared to other analytical tools, including the ability to perform label-free and fluid phase imaging which allows researchers to visualize, probe, and analyze intricate structures of biological molecules in their native environments. In addition, binding and intermolecular interactions between two biomolecules can be studied using the AFM, making it a very powerful tool for investigating the biomolecular nano-assemblies. This chapter focuses on the application of AFM in neurodegenerative disease studies. We show how AFM can be used to characterize protein structures at various aggregation stages; to detect changes in conformations of proteins and protein-antibody complexes, to determine the binding interactions between an antibody and antigen and to quantify binding specificity of an antibody to its antigen. In addition, we highlight the development of novel AFM techniques to facilitate the study of protein morphologies and interactions in neuroscience research. We hope that this chapter offers new insights and appreciation to the reader on the versatility of the AFM in nanotechnology research, particularly nanobioscience.

Original languageEnglish (US)
Title of host publicationNanomedicine and the Nervous System
PublisherCRC Press
Pages75-96
Number of pages22
ISBN (Electronic)9781466505117
ISBN (Print)9781578087280
StatePublished - Jan 1 2012

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Neurodegenerative diseases
Atomic Force Microscopy
Neurodegenerative Diseases
Atomic force microscopy
Proteins
Antibodies
Antigens
Protein Conformation
Nanotechnology
Antibody Specificity
Biomolecules
Neurosciences
Research
Conformations
Labels
Agglomeration
Research Personnel
Imaging techniques
Molecules
Fluids

ASJC Scopus subject areas

  • Medicine(all)
  • Engineering(all)

Cite this

Wang, M. S., & Sierks, M. (2012). Probing protein nanoassemblies in neurodegenerative disease. In Nanomedicine and the Nervous System (pp. 75-96). CRC Press.

Probing protein nanoassemblies in neurodegenerative disease. / Wang, Min S.; Sierks, Michael.

Nanomedicine and the Nervous System. CRC Press, 2012. p. 75-96.

Research output: Chapter in Book/Report/Conference proceedingChapter

Wang, MS & Sierks, M 2012, Probing protein nanoassemblies in neurodegenerative disease. in Nanomedicine and the Nervous System. CRC Press, pp. 75-96.
Wang MS, Sierks M. Probing protein nanoassemblies in neurodegenerative disease. In Nanomedicine and the Nervous System. CRC Press. 2012. p. 75-96
Wang, Min S. ; Sierks, Michael. / Probing protein nanoassemblies in neurodegenerative disease. Nanomedicine and the Nervous System. CRC Press, 2012. pp. 75-96
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