Prevalent and distinct spliceosomal 3′-end processing mechanisms for fungal telomerase RNA

Xiaodong Qi, Dustin P. Rand, Joshua D. Podlevsky, Yang Li, Axel Mosig, Peter F. Stadler, Julian Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Telomerase RNA (TER) is an essential component of the telomerase ribonucleoprotein complex. The mechanism for TER 3′-end processing is highly divergent among different organisms. Here we report a unique spliceosome-mediated TER 3′-end cleavage mechanism in Neurospora crassa that is distinct from that found specifically in the fission yeast Schizosaccharomyces pombe. While the S. pombe TER intron contains the canonical 5′-splice site GUAUGU, the N. crassa TER intron contains a non-canonical 5′-splice site AUAAGU that alone prevents the second step of splicing and promotes spliceosomal cleavage. The unique N. crassa TER 5′-splice site sequence is evolutionarily conserved in TERs from Pezizomycotina and early branching Taphrinomycotina species. This suggests that the widespread and basal N. crassa-type spliceosomal cleavage mechanism is more ancestral than the S. pombe-type. The discovery of a prevalent, yet distinct, spliceosomal cleavage mechanism throughout diverse fungal clades furthers our understanding of TER evolution and non-coding RNA processing.

Original languageEnglish (US)
Article number6105
JournalNature Communications
Volume6
DOIs
StatePublished - 2015

Fingerprint

Fungal RNA
cleavage
Neurospora crassa
RNA Splice Sites
Schizosaccharomyces
Processing
neurospora
splicing
yeast
RNA 3' End Processing
Introns
organisms
fission
Spliceosomes
Untranslated RNA
Ribonucleoproteins
Telomerase
telomerase RNA
Yeast

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Prevalent and distinct spliceosomal 3′-end processing mechanisms for fungal telomerase RNA. / Qi, Xiaodong; Rand, Dustin P.; Podlevsky, Joshua D.; Li, Yang; Mosig, Axel; Stadler, Peter F.; Chen, Julian.

In: Nature Communications, Vol. 6, 6105, 2015.

Research output: Contribution to journalArticle

Qi, Xiaodong ; Rand, Dustin P. ; Podlevsky, Joshua D. ; Li, Yang ; Mosig, Axel ; Stadler, Peter F. ; Chen, Julian. / Prevalent and distinct spliceosomal 3′-end processing mechanisms for fungal telomerase RNA. In: Nature Communications. 2015 ; Vol. 6.
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