Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates

Alexander N. Patananan; Alexander J. Sercel; Ting Hsiang Wu; Fasih M. Ahsan; Alejandro Torres; Stephanie A.L. Kennedy; Amy Vandiver; Amanda J. Collier; Artin Mehrabi; Jon Van Lew; Lise Zakin; Noe Rodriguez; Marcos Sixto; Wael Tadros; Adam Lazar; Peter A. Sieling; Thang L. Nguyen; Emma R. Dawson; Daniel Braas; Justin Golovato; Luis Cisneros; Charles Vaske; Kathrin Plath; Shahrooz Rabizadeh; Kayvan R. Niazi; Pei Yu Chiou; Michael A. Teitell

doi:10.1016/j.celrep.2020.108562

Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates

Alexander N. Patananan, Alexander J. Sercel, Ting Hsiang Wu, Fasih M. Ahsan, Alejandro Torres, Stephanie A.L. Kennedy, Amy Vandiver, Amanda J. Collier, Artin Mehrabi, Jon Van Lew, Lise Zakin, Noe Rodriguez, Marcos Sixto, Wael Tadros, Adam Lazar, Peter A. Sieling, Thang L. Nguyen, Emma R. Dawson, Daniel Braas, Justin GolovatoLuis Cisneros, Charles Vaske, Kathrin Plath, Shahrooz Rabizadeh, Kayvan R. Niazi, Pei Yu Chiou, Michael A. Teitell

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Patananan and colleagues demonstrate a pipeline for transferring isolated mitochondria into mtDNA-deficient recipient cells. mtDNA-depleted fibroblasts permanently retain acquired non-native mtDNA through cell fate transitions. Initially, mitochondrial recipients show mtDNA-deficient cell transcriptome and metabolome profiles, with improvement to control profiles by reprogramming to pluripotency and subsequent differentiation.

Original language	English (US)
Article number	108562
Journal	Cell Reports
Volume	33
Issue number	13
DOIs	https://doi.org/10.1016/j.celrep.2020.108562
State	Published - Dec 29 2020
Externally published	Yes

Keywords

cell engineering
differentiation, MitoPunch, mitochondrial transplantation, mitochondrial replacement, mitonuclear communication, isolated mitochondria
mitochondrial transfer
mtDNA
reprogramming

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2020.108562

Cite this

Patananan, A. N., Sercel, A. J., Wu, T. H., Ahsan, F. M., Torres, A., Kennedy, S. A. L., Vandiver, A., Collier, A. J., Mehrabi, A., Van Lew, J., Zakin, L., Rodriguez, N., Sixto, M., Tadros, W., Lazar, A., Sieling, P. A., Nguyen, T. L., Dawson, E. R., Braas, D., ... Teitell, M. A. (2020). Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates. Cell Reports, 33(13), Article 108562. https://doi.org/10.1016/j.celrep.2020.108562

Patananan, AN, Sercel, AJ, Wu, TH, Ahsan, FM, Torres, A, Kennedy, SAL, Vandiver, A, Collier, AJ, Mehrabi, A, Van Lew, J, Zakin, L, Rodriguez, N, Sixto, M, Tadros, W, Lazar, A, Sieling, PA, Nguyen, TL, Dawson, ER, Braas, D, Golovato, J, Cisneros, L, Vaske, C, Plath, K, Rabizadeh, S, Niazi, KR, Chiou, PY & Teitell, MA 2020, 'Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates', Cell Reports, vol. 33, no. 13, 108562. https://doi.org/10.1016/j.celrep.2020.108562

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title = "Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates",

abstract = "Patananan and colleagues demonstrate a pipeline for transferring isolated mitochondria into mtDNA-deficient recipient cells. mtDNA-depleted fibroblasts permanently retain acquired non-native mtDNA through cell fate transitions. Initially, mitochondrial recipients show mtDNA-deficient cell transcriptome and metabolome profiles, with improvement to control profiles by reprogramming to pluripotency and subsequent differentiation.",

keywords = "cell engineering, differentiation, MitoPunch, mitochondrial transplantation, mitochondrial replacement, mitonuclear communication, isolated mitochondria, mitochondrial transfer, mtDNA, reprogramming",

author = "Patananan, {Alexander N.} and Sercel, {Alexander J.} and Wu, {Ting Hsiang} and Ahsan, {Fasih M.} and Alejandro Torres and Kennedy, {Stephanie A.L.} and Amy Vandiver and Collier, {Amanda J.} and Artin Mehrabi and {Van Lew}, Jon and Lise Zakin and Noe Rodriguez and Marcos Sixto and Wael Tadros and Adam Lazar and Sieling, {Peter A.} and Nguyen, {Thang L.} and Dawson, {Emma R.} and Daniel Braas and Justin Golovato and Luis Cisneros and Charles Vaske and Kathrin Plath and Shahrooz Rabizadeh and Niazi, {Kayvan R.} and Chiou, {Pei Yu} and Teitell, {Michael A.}",

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AU - Patananan, Alexander N.

AU - Sercel, Alexander J.

AU - Wu, Ting Hsiang

AU - Ahsan, Fasih M.

AU - Torres, Alejandro

AU - Kennedy, Stephanie A.L.

AU - Vandiver, Amy

AU - Collier, Amanda J.

AU - Mehrabi, Artin

AU - Van Lew, Jon

AU - Zakin, Lise

AU - Rodriguez, Noe

AU - Sixto, Marcos

AU - Tadros, Wael

AU - Lazar, Adam

AU - Sieling, Peter A.

AU - Nguyen, Thang L.

AU - Dawson, Emma R.

AU - Braas, Daniel

AU - Golovato, Justin

AU - Cisneros, Luis

AU - Vaske, Charles

AU - Plath, Kathrin

AU - Rabizadeh, Shahrooz

AU - Niazi, Kayvan R.

AU - Chiou, Pei Yu

AU - Teitell, Michael A.

PY - 2020/12/29

Y1 - 2020/12/29

N2 - Patananan and colleagues demonstrate a pipeline for transferring isolated mitochondria into mtDNA-deficient recipient cells. mtDNA-depleted fibroblasts permanently retain acquired non-native mtDNA through cell fate transitions. Initially, mitochondrial recipients show mtDNA-deficient cell transcriptome and metabolome profiles, with improvement to control profiles by reprogramming to pluripotency and subsequent differentiation.

AB - Patananan and colleagues demonstrate a pipeline for transferring isolated mitochondria into mtDNA-deficient recipient cells. mtDNA-depleted fibroblasts permanently retain acquired non-native mtDNA through cell fate transitions. Initially, mitochondrial recipients show mtDNA-deficient cell transcriptome and metabolome profiles, with improvement to control profiles by reprogramming to pluripotency and subsequent differentiation.

KW - cell engineering

KW - differentiation, MitoPunch, mitochondrial transplantation, mitochondrial replacement, mitonuclear communication, isolated mitochondria

KW - mitochondrial transfer

KW - mtDNA

KW - reprogramming

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Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates

Abstract

Keywords

ASJC Scopus subject areas

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