@article{a8ea3f606ce64c569cbc2847ccbc0893,
title = "Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates",
abstract = "Patananan and colleagues demonstrate a pipeline for transferring isolated mitochondria into mtDNA-deficient recipient cells. mtDNA-depleted fibroblasts permanently retain acquired non-native mtDNA through cell fate transitions. Initially, mitochondrial recipients show mtDNA-deficient cell transcriptome and metabolome profiles, with improvement to control profiles by reprogramming to pluripotency and subsequent differentiation.",
keywords = "cell engineering, differentiation, MitoPunch, mitochondrial transplantation, mitochondrial replacement, mitonuclear communication, isolated mitochondria, mitochondrial transfer, mtDNA, reprogramming",
author = "Patananan, {Alexander N.} and Sercel, {Alexander J.} and Wu, {Ting Hsiang} and Ahsan, {Fasih M.} and Alejandro Torres and Kennedy, {Stephanie A.L.} and Amy Vandiver and Collier, {Amanda J.} and Artin Mehrabi and {Van Lew}, Jon and Lise Zakin and Noe Rodriguez and Marcos Sixto and Wael Tadros and Adam Lazar and Sieling, {Peter A.} and Nguyen, {Thang L.} and Dawson, {Emma R.} and Daniel Braas and Justin Golovato and Luis Cisneros and Charles Vaske and Kathrin Plath and Shahrooz Rabizadeh and Niazi, {Kayvan R.} and Chiou, {Pei Yu} and Teitell, {Michael A.}",
note = "Funding Information: A.N.P. is supported by the NIH ( T32CA009120 ) and American Heart Association ( 18POST34080342 ). A.J.S. is supported by the NIH ( T32GM007185 and T32CA009120 ). K.P. is supported by the Broad Center of Regenerative Medicine and Stem Cell Research at UCLA , the David Geffen School of Medicine , the NIH ( P01GM099134 ), and a Faculty Scholar grant from the HHMI . M.A.T. is supported by the Air Force Office of Scientific Research ( FA9550-15-1-0406 ), the NIH ( R01GM114188 , R01GM073981 , R01CA185189 , R21CA227480 , R01GM127985 , and P30CA016042 ), and CIRM ( RT3-07678 ). We thank Rebeca Acin-Perez, Linsey Stiles, and Orian Shirihai of the UCLA Metabolomics Core for help with Seahorse XF Analyzer assays. We thank Felicia Codrea from the UCLA Flow Cytometry Core, in addition to Laurent Bentolila and Matthew Schibler from the California Nanosystems Institute Advanced Light Microscopy/Spectroscopy Laboratory for their insight and assistance. We thank Jonathan Wanagat for his critical insights during manuscript preparation. We also acknowledge Drs. Idoya Lahortiga and Luk Cox for their Library of Science and Medical Illustrations ( https://www.somersault1824.com/ ). Funding Information: A.N.P. is supported by the NIH (T32CA009120) and American Heart Association (18POST34080342). A.J.S. is supported by the NIH (T32GM007185 and T32CA009120). K.P. is supported by the Broad Center of Regenerative Medicine and Stem Cell Research at UCLA, the David Geffen School of Medicine, the NIH (P01GM099134), and a Faculty Scholar grant from the HHMI. M.A.T. is supported by the Air Force Office of Scientific Research (FA9550-15-1-0406), the NIH (R01GM114188, R01GM073981, R01CA185189, R21CA227480, R01GM127985, and P30CA016042), and CIRM (RT3-07678). We thank Rebeca Acin-Perez, Linsey Stiles, and Orian Shirihai of the UCLA Metabolomics Core for help with Seahorse XF Analyzer assays. We thank Felicia Codrea from the UCLA Flow Cytometry Core, in addition to Laurent Bentolila and Matthew Schibler from the California Nanosystems Institute Advanced Light Microscopy/Spectroscopy Laboratory for their insight and assistance. We thank Jonathan Wanagat for his critical insights during manuscript preparation. We also acknowledge Drs. Idoya Lahortiga and Luk Cox for their Library of Science and Medical Illustrations (https://www.somersault1824.com/). Conceptualization: A.N.P. A.J.S. T.-H.W. S.R. K.R.N. and M.A.T.; Methodology: A.N.P. A.J.S. T.-H.W. F.M.A. A.M. J.V.L. S.R. K.R.N. P.-Y.C. and M.A.T.; Software: T.L.N. F.M.A. A.V. J.G. and D.B.; Formal Analysis: A.N.P. A.J.S. F.M.A. A.V. T.L.N. and D.B.; Investigation: A.N.P. A.J.S. T.-H.W. F.M.A. A.V. S.A.L.K. T.N. A.T. A.J.C. E.R.D. A.M. J.V.L. L.Z. N.R. M.S. W.T. A.L. P.A.S. D.B. J.G. and C.V.; Resources: A.N.P. A.J.S. T.-H.W. and F.M.A.; Data Curation: A.N.P. F.M.A. A.V. D.B. and C.V.; Writing ? Original Draft: A.N.P. A.J.S. and F.M.A.; Writing ? Review & Editing: A.N.P. A.J.S. F.M.A. A.V. T.-H.W. K.R.N. and M.A.T.; Validation: A.N.P. and A.J.S.; Visualization: A.N.P. A.J.S. T.L.N. F.M.A. and A.V.; Supervision: A.N.P. and T.-H.W.; Project Administration: A.N.P. and T.-H.W.; Funding Acquisition: A.N.P. A.J.S. S.R. K.R.N. P.-Y.C. K.P. and M.A.T. M.A.T. and P.-Y.C. are co-founders, board members, shareholders, and consultants for NanoCav, a private start-up company working on mitochondrial transfer and quantitative phase microscopy techniques and applications. T.-H.W. was an employee of NanoCav and is employed by ImmunityBio, and S.R. and K.R.N. are board members of NanoCav and employed by ImmunityBio. The other authors report no competing interests. Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = dec,
day = "29",
doi = "10.1016/j.celrep.2020.108562",
language = "English (US)",
volume = "33",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "13",
}