Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates

Alexander N. Patananan, Alexander J. Sercel, Ting Hsiang Wu, Fasih M. Ahsan, Alejandro Torres, Stephanie A.L. Kennedy, Amy Vandiver, Amanda J. Collier, Artin Mehrabi, Jon Van Lew, Lise Zakin, Noe Rodriguez, Marcos Sixto, Wael Tadros, Adam Lazar, Peter A. Sieling, Thang L. Nguyen, Emma R. Dawson, Daniel Braas, Justin GolovatoLuis Cisneros, Charles Vaske, Kathrin Plath, Shahrooz Rabizadeh, Kayvan R. Niazi, Pei Yu Chiou, Michael A. Teitell

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Patananan and colleagues demonstrate a pipeline for transferring isolated mitochondria into mtDNA-deficient recipient cells. mtDNA-depleted fibroblasts permanently retain acquired non-native mtDNA through cell fate transitions. Initially, mitochondrial recipients show mtDNA-deficient cell transcriptome and metabolome profiles, with improvement to control profiles by reprogramming to pluripotency and subsequent differentiation.

Original languageEnglish (US)
Article number108562
JournalCell Reports
Volume33
Issue number13
DOIs
StatePublished - Dec 29 2020
Externally publishedYes

Keywords

  • cell engineering
  • differentiation, MitoPunch, mitochondrial transplantation, mitochondrial replacement, mitonuclear communication, isolated mitochondria
  • mitochondrial transfer
  • mtDNA
  • reprogramming

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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