Pressure-assisted electrokinetic supercharging for the enhancement of non-steroidal anti-inflammatory drugs

Michelle M. Meighan, Mohamed Dawod, Rosanne M. Guijt, Mark Hayes, Michael C. Breadmore

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.

Original languageEnglish (US)
Pages (from-to)6750-6755
Number of pages6
JournalJournal of Chromatography A
Volume1218
Issue number38
DOIs
StatePublished - Sep 23 2011

Keywords

  • Capillary electrophoresis
  • Electrokinetic supercharging
  • Non-steroidal anti-inflammatory drugs
  • Pressure-assisted
  • Stacking
  • Water samples

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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