TY - JOUR
T1 - Pressure-assisted electrokinetic supercharging for the enhancement of non-steroidal anti-inflammatory drugs
AU - Meighan, Michelle M.
AU - Dawod, Mohamed
AU - Guijt, Rosanne M.
AU - Hayes, Mark
AU - Breadmore, Michael C.
N1 - Funding Information:
M.M.M. would like to acknowledge the National Science Foundation East Asia and Pacific Summer Institutes and the Australian Academy of Sciences for support for this research. M.C.B would like to acknowledge the Australian Research Council for funding and provision of a QEII Fellowship (DP0984745).
PY - 2011/9/23
Y1 - 2011/9/23
N2 - Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.
AB - Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.
KW - Capillary electrophoresis
KW - Electrokinetic supercharging
KW - Non-steroidal anti-inflammatory drugs
KW - Pressure-assisted
KW - Stacking
KW - Water samples
UR - http://www.scopus.com/inward/record.url?scp=80052100824&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052100824&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2011.07.044
DO - 10.1016/j.chroma.2011.07.044
M3 - Article
C2 - 21855878
AN - SCOPUS:80052100824
VL - 1218
SP - 6750
EP - 6755
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 38
ER -