Abstract
Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6750-6755 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 1218 |
| Issue number | 38 |
| DOIs | |
| State | Published - Sep 23 2011 |
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Keywords
- Capillary electrophoresis
- Electrokinetic supercharging
- Non-steroidal anti-inflammatory drugs
- Pressure-assisted
- Stacking
- Water samples
ASJC Scopus subject areas
- Analytical Chemistry
- Organic Chemistry
- Biochemistry
Cite this
Pressure-assisted electrokinetic supercharging for the enhancement of non-steroidal anti-inflammatory drugs. / Meighan, Michelle M.; Dawod, Mohamed; Guijt, Rosanne M.; Hayes, Mark; Breadmore, Michael C.
In: Journal of Chromatography A, Vol. 1218, No. 38, 23.09.2011, p. 6750-6755.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pressure-assisted electrokinetic supercharging for the enhancement of non-steroidal anti-inflammatory drugs
AU - Meighan, Michelle M.
AU - Dawod, Mohamed
AU - Guijt, Rosanne M.
AU - Hayes, Mark
AU - Breadmore, Michael C.
PY - 2011/9/23
Y1 - 2011/9/23
N2 - Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.
AB - Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.
KW - Capillary electrophoresis
KW - Electrokinetic supercharging
KW - Non-steroidal anti-inflammatory drugs
KW - Pressure-assisted
KW - Stacking
KW - Water samples
UR - http://www.scopus.com/inward/record.url?scp=80052100824&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052100824&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2011.07.044
DO - 10.1016/j.chroma.2011.07.044
M3 - Article
VL - 1218
SP - 6750
EP - 6755
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 38
ER -