Pressure-assisted electrokinetic supercharging for the enhancement of non-steroidal anti-inflammatory drugs

Michelle M. Meighan; Mohamed Dawod; Rosanne M. Guijt; Mark Hayes; Michael C. Breadmore

doi:10.1016/j.chroma.2011.07.044

Pressure-assisted electrokinetic supercharging for the enhancement of non-steroidal anti-inflammatory drugs

Michelle M. Meighan, Mohamed Dawod, Rosanne M. Guijt, Mark Hayes, Michael C. Breadmore

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.

Original language	English (US)
Pages (from-to)	6750-6755
Number of pages	6
Journal	Journal of Chromatography A
Volume	1218
Issue number	38
DOIs	https://doi.org/10.1016/j.chroma.2011.07.044
State	Published - Sep 23 2011

Keywords

Capillary electrophoresis
Electrokinetic supercharging
Non-steroidal anti-inflammatory drugs
Pressure-assisted
Stacking
Water samples

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2011.07.044

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title = "Pressure-assisted electrokinetic supercharging for the enhancement of non-steroidal anti-inflammatory drugs",

abstract = "Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.",

keywords = "Capillary electrophoresis, Electrokinetic supercharging, Non-steroidal anti-inflammatory drugs, Pressure-assisted, Stacking, Water samples",

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AU - Meighan, Michelle M.

AU - Dawod, Mohamed

AU - Guijt, Rosanne M.

AU - Hayes, Mark

AU - Breadmore, Michael C.

PY - 2011/9/23

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N2 - Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.

AB - Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.

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KW - Pressure-assisted

KW - Stacking

KW - Water samples

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