Presence of lesions without virus replication in the thymus of chickens exposed to infectious bursal disease virus.

J. M. Sharma, J. Dohms, M. Walser, D. B. Snyder

Research output: Contribution to journalArticle

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Abstract

Specific-pathogen-free (SPF) chickens were exposed to the IM and VA isolates of virulent infectious bursal disease virus (IBDV). Both viruses induced rapidly progressing lymphoid cell depletion in the bursa. The bursal lesions persisted through the observation period of 16 days. The virus-exposed birds also had histologic lesions in the thymus. Thymic lesions peaked at 3-4 days postinoculation (PI) and then subsided. Immunofluorescence (IF) and antigen-capture enzyme-linked immunosorbent assay (ELISA) detected abundant viral antigen in the bursa, but not in the thymus, of chickens during the first week after infection with IM-IBDV or VA-IBDV. This result indicated that the presence of histologic lesions in the thymus was not associated with active infection and replication of the virus in thymic cells. Inoculation of homogenates of bursal and thymic tissues from virus-exposed chickens into embryonated chicken eggs revealed the presence of infectious virus from both tissues. We speculated that the virus recovered from thymus may have been contributed by virus-infected cells that were circulating through the thymus at the time when this tissue was homogenized.

Original languageEnglish (US)
Pages (from-to)741-748
Number of pages8
JournalAvian Diseases
Volume37
Issue number3
StatePublished - Jul 1993
Externally publishedYes

Fingerprint

Infectious bursal disease virus
Virus Replication
virus replication
lesions (animal)
Thymus Gland
Chickens
chickens
Viruses
viruses
Specific Pathogen-Free Organisms
Viral Antigens
chicken eggs
viral antigens
Virus Diseases
cells
infection
Eggs
fluorescent antibody technique
Birds
Fluorescent Antibody Technique

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • veterinary(all)

Cite this

Presence of lesions without virus replication in the thymus of chickens exposed to infectious bursal disease virus. / Sharma, J. M.; Dohms, J.; Walser, M.; Snyder, D. B.

In: Avian Diseases, Vol. 37, No. 3, 07.1993, p. 741-748.

Research output: Contribution to journalArticle

Sharma, J. M. ; Dohms, J. ; Walser, M. ; Snyder, D. B. / Presence of lesions without virus replication in the thymus of chickens exposed to infectious bursal disease virus. In: Avian Diseases. 1993 ; Vol. 37, No. 3. pp. 741-748.
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