Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated "chemical aminoacylation"

T. G. Heckler, L. H. Chang, Y. Zama, T. Naka, S. M. Hecht

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


The synthesis of '2('3)-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of E. coli tRNA phe-COH. The modified (N-acetylammo)acyl-tRNA's so derived are characterized by their chromatographic and biochemical properties, and by their ability to act as P-site tRNA donors in the peptidyltransferase reaction.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
Issue number1
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry


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