Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated "chemical aminoacylation"

T. G. Heckler; L. H. Chang; Y. Zama; T. Naka; S. M. Hecht

doi:10.1016/0040-4020(84)85106-6

Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated "chemical aminoacylation"

T. G. Heckler, L. H. Chang, Y. Zama, T. Naka, S. M. Hecht

Research output: Contribution to journal › Article › peer-review

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Abstract

The synthesis of '2('3)-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of E. coli tRNA ^phe-C_OH. The modified (N-acetylammo)acyl-tRNA's so derived are characterized by their chromatographic and biochemical properties, and by their ability to act as P-site tRNA donors in the peptidyltransferase reaction.

Original language	English (US)
Pages (from-to)	87-94
Number of pages	8
Journal	Tetrahedron
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/0040-4020(84)85106-6
State	Published - 1984
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Drug Discovery
Organic Chemistry

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10.1016/0040-4020(84)85106-6

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title = "Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated {"}chemical aminoacylation{"}",

abstract = "The synthesis of '2('3)-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of E. coli tRNA phe-COH. The modified (N-acetylammo)acyl-tRNA's so derived are characterized by their chromatographic and biochemical properties, and by their ability to act as P-site tRNA donors in the peptidyltransferase reaction.",

author = "Heckler, {T. G.} and Chang, {L. H.} and Y. Zama and T. Naka and Hecht, {S. M.}",

note = "Funding Information: Acknowledgement-This work was supportedb y a grant from the National ScienceF oundation( PCM79-23063).",

year = "1984",

doi = "10.1016/0040-4020(84)85106-6",

language = "English (US)",

volume = "40",

pages = "87--94",

journal = "Tetrahedron",

issn = "0040-4020",

publisher = "Elsevier Limited",

number = "1",

}

TY - JOUR

T1 - Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated "chemical aminoacylation"

AU - Heckler, T. G.

AU - Chang, L. H.

AU - Zama, Y.

AU - Naka, T.

AU - Hecht, S. M.

N1 - Funding Information: Acknowledgement-This work was supportedb y a grant from the National ScienceF oundation( PCM79-23063).

PY - 1984

Y1 - 1984

N2 - The synthesis of '2('3)-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of E. coli tRNA phe-COH. The modified (N-acetylammo)acyl-tRNA's so derived are characterized by their chromatographic and biochemical properties, and by their ability to act as P-site tRNA donors in the peptidyltransferase reaction.

AB - The synthesis of '2('3)-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of E. coli tRNA phe-COH. The modified (N-acetylammo)acyl-tRNA's so derived are characterized by their chromatographic and biochemical properties, and by their ability to act as P-site tRNA donors in the peptidyltransferase reaction.

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UR - http://www.scopus.com/inward/citedby.url?scp=0002776027&partnerID=8YFLogxK

U2 - 10.1016/0040-4020(84)85106-6

DO - 10.1016/0040-4020(84)85106-6

M3 - Article

AN - SCOPUS:0002776027

SN - 0040-4020

VL - 40

SP - 87

EP - 94

JO - Tetrahedron

JF - Tetrahedron

IS - 1

ER -

Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated "chemical aminoacylation"

Abstract

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