Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated "chemical aminoacylation"

T. G. Heckler, L. H. Chang, Y. Zama, T. Naka, Sidney Hecht

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The synthesis of '2('3)-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of E. coli tRNA phe-COH. The modified (N-acetylammo)acyl-tRNA's so derived are characterized by their chromatographic and biochemical properties, and by their ability to act as P-site tRNA donors in the peptidyltransferase reaction.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalTetrahedron
Volume40
Issue number1
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Aminoacylation
Ligases
Transfer RNA
RNA
RNA, Transfer, Phe
Peptidyl Transferases
Derivatives
Substrates
Escherichia coli

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Drug Discovery

Cite this

Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated "chemical aminoacylation". / Heckler, T. G.; Chang, L. H.; Zama, Y.; Naka, T.; Hecht, Sidney.

In: Tetrahedron, Vol. 40, No. 1, 1984, p. 87-94.

Research output: Contribution to journalArticle

Heckler, T. G. ; Chang, L. H. ; Zama, Y. ; Naka, T. ; Hecht, Sidney. / Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated "chemical aminoacylation". In: Tetrahedron. 1984 ; Vol. 40, No. 1. pp. 87-94.
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