Preparation and evaluation of a radioisotope-incorporated iron oxide core/Au shell nanoplatform for dual modality imaging

You Fu Zhou, Praveen Gulaka, Jian Zhou, Ming Xiao, Dongsheng Xu, Jer Tsong Hsieh, Vikram Kodibagkar, Xiankai Sun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Magnetic gold-coated iron oxide nanoparticles (FeΑu NPs) have recently emerged as a new type of iron oxide nanoparticle based magnetic resonance imaging (MRI) contrast agents, in which the gold shell provides a conveniently tunable surface for the presentation of multiple functional molecules. Given the versatility of this nanoplatform and the intrinsic sensitivity limitation of MRI contrast agents, a new approach was developed in this work to incorporate radioisotopes with suitable half-lives into the iron oxide core of FeΑu NPs and impart the superior sensitivity of nuclear imaging to the nanoplatform. The incorporation of 67Ga was successfully accomplished by co-precipitation of 67Ga3+ with Fe3+/Fe2+ ions at a pH ranging from 4-10. The gold coating procedure was carried out by an iterative hydroxylamine seeding process. Upon the gold deposition, the hydrodynamic radius of the nanoparticles was changed from 23.2±2.2 nm to 31.7 ± 2.3 nm, indicating an 8-nm thickness for the gold shell. The FeΑu NPs were functionalized by lipoic acid (LA) and further conjugated with a polyarginine cell permeation peptide, NH2GR11. All the FeΑu NPs stayed nearly 100% intact in either PBS or rat serum within 72 h. Cell labeling with the LA-modified FeΑu NPs and NH2GR11-conjugated FeΑu NPs was conducted by using a human prostate cancer cell line (PC-3). It was shown that NH2GR11 was able to increase the nanoparticle loading to PC-3 cells by 2-3 times. Shown in a pilot dual-modality imaging study, the LA-modified FeΑu NP labeled cells could be visualized by both MRI and autoradiography imaging if the labeled PC-3 cell concentrations were above 1 × 105 cells/mL. The cell permeation peptide, NH2GR11, could significantly enhance the dual-modality detection sensitivity of the nanoplatform labeled PC-3 cells.

Original languageEnglish (US)
Pages (from-to)474-481
Number of pages8
JournalJournal of Biomedical Nanotechnology
Volume4
Issue number4
DOIs
StatePublished - Dec 2008
Externally publishedYes

Fingerprint

Iron oxides
Radioisotopes
Imaging techniques
Thioctic Acid
Gold
Magnetic resonance
Nanoparticles
Permeation
Contrast Media
Peptides
Acids
Magnetic Resonance Imaging
Gold coatings
Hydroxylamine
Coprecipitation
Labeling
ferric oxide
Rats
Hydrodynamics
Autoradiography

Keywords

  • Cell trafficking
  • Gold nanoparticles
  • MRI
  • Nuclear imaging
  • Prostate cancer
  • Superparamagnetic iron oxide nanoparticles (SPIO)

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

Preparation and evaluation of a radioisotope-incorporated iron oxide core/Au shell nanoplatform for dual modality imaging. / Zhou, You Fu; Gulaka, Praveen; Zhou, Jian; Xiao, Ming; Xu, Dongsheng; Hsieh, Jer Tsong; Kodibagkar, Vikram; Sun, Xiankai.

In: Journal of Biomedical Nanotechnology, Vol. 4, No. 4, 12.2008, p. 474-481.

Research output: Contribution to journalArticle

Zhou, You Fu ; Gulaka, Praveen ; Zhou, Jian ; Xiao, Ming ; Xu, Dongsheng ; Hsieh, Jer Tsong ; Kodibagkar, Vikram ; Sun, Xiankai. / Preparation and evaluation of a radioisotope-incorporated iron oxide core/Au shell nanoplatform for dual modality imaging. In: Journal of Biomedical Nanotechnology. 2008 ; Vol. 4, No. 4. pp. 474-481.
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