Preparation and characterization of porous gold and its application as a platform for immobilization of acetylcholine esterase

Olga V. Shulga, Kenise Jefferson, Abdul R. Khan, Valerian T. D'Souza, Jingyue Liu, Alexei V. Demchenko, Keith J. Stine

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

We report a method for the fabrication of a free-standing porous gold material with a high surface area and well-defined, tunable pore morphology. Porous gold is formed via a simple procedure that involves acidic treatment of a commercially available complex white gold alloy. We used SEM and AFM techniques to characterize the surface morphology, size, and shape of the mesopores as well as the surface roughness of the prepared porous gold samples. Formation of self-assembled monolayers of a flavin sulfide on the gold surface was used to estimate the total surface area of the porous gold material. The monolayers were found to be electrochemically active by cyclic and square wave voltammetry. It was found that 24 h HNO3 treatment gave a 12 400× surface enlargement and resulted in a surface area of 14.2 m2/g, whereas 72 h HNO3 treatment resulted in a 6900× surface enlargement and a surface area of 8.2 m2/g. In addition, the enzyme acetylcholine esterase was immobilized on the different porous gold surfaces to demonstrate biocompatibility of the porous gold material. Kinetic parameters and the amount of the immobilized acetylcholine esterase were determined.

Original languageEnglish (US)
Pages (from-to)3902-3911
Number of pages10
JournalChemistry of Materials
Volume19
Issue number16
DOIs
StatePublished - Aug 7 2007
Externally publishedYes

Fingerprint

Esterases
Gold
Acetylcholine
Gold Alloys
Gold alloys
Self assembled monolayers
Sulfides
Voltammetry
Biocompatibility
Kinetic parameters
Surface morphology
Monolayers
Enzymes
Surface roughness
Fabrication
Scanning electron microscopy

ASJC Scopus subject areas

  • Materials Chemistry
  • Materials Science(all)

Cite this

Preparation and characterization of porous gold and its application as a platform for immobilization of acetylcholine esterase. / Shulga, Olga V.; Jefferson, Kenise; Khan, Abdul R.; D'Souza, Valerian T.; Liu, Jingyue; Demchenko, Alexei V.; Stine, Keith J.

In: Chemistry of Materials, Vol. 19, No. 16, 07.08.2007, p. 3902-3911.

Research output: Contribution to journalArticle

Shulga, Olga V. ; Jefferson, Kenise ; Khan, Abdul R. ; D'Souza, Valerian T. ; Liu, Jingyue ; Demchenko, Alexei V. ; Stine, Keith J. / Preparation and characterization of porous gold and its application as a platform for immobilization of acetylcholine esterase. In: Chemistry of Materials. 2007 ; Vol. 19, No. 16. pp. 3902-3911.
@article{25ce0573624b409e884d758198af54b8,
title = "Preparation and characterization of porous gold and its application as a platform for immobilization of acetylcholine esterase",
abstract = "We report a method for the fabrication of a free-standing porous gold material with a high surface area and well-defined, tunable pore morphology. Porous gold is formed via a simple procedure that involves acidic treatment of a commercially available complex white gold alloy. We used SEM and AFM techniques to characterize the surface morphology, size, and shape of the mesopores as well as the surface roughness of the prepared porous gold samples. Formation of self-assembled monolayers of a flavin sulfide on the gold surface was used to estimate the total surface area of the porous gold material. The monolayers were found to be electrochemically active by cyclic and square wave voltammetry. It was found that 24 h HNO3 treatment gave a 12 400× surface enlargement and resulted in a surface area of 14.2 m2/g, whereas 72 h HNO3 treatment resulted in a 6900× surface enlargement and a surface area of 8.2 m2/g. In addition, the enzyme acetylcholine esterase was immobilized on the different porous gold surfaces to demonstrate biocompatibility of the porous gold material. Kinetic parameters and the amount of the immobilized acetylcholine esterase were determined.",
author = "Shulga, {Olga V.} and Kenise Jefferson and Khan, {Abdul R.} and D'Souza, {Valerian T.} and Jingyue Liu and Demchenko, {Alexei V.} and Stine, {Keith J.}",
year = "2007",
month = "8",
day = "7",
doi = "10.1021/cm070238n",
language = "English (US)",
volume = "19",
pages = "3902--3911",
journal = "Chemistry of Materials",
issn = "0897-4756",
publisher = "American Chemical Society",
number = "16",

}

TY - JOUR

T1 - Preparation and characterization of porous gold and its application as a platform for immobilization of acetylcholine esterase

AU - Shulga, Olga V.

AU - Jefferson, Kenise

AU - Khan, Abdul R.

AU - D'Souza, Valerian T.

AU - Liu, Jingyue

AU - Demchenko, Alexei V.

AU - Stine, Keith J.

PY - 2007/8/7

Y1 - 2007/8/7

N2 - We report a method for the fabrication of a free-standing porous gold material with a high surface area and well-defined, tunable pore morphology. Porous gold is formed via a simple procedure that involves acidic treatment of a commercially available complex white gold alloy. We used SEM and AFM techniques to characterize the surface morphology, size, and shape of the mesopores as well as the surface roughness of the prepared porous gold samples. Formation of self-assembled monolayers of a flavin sulfide on the gold surface was used to estimate the total surface area of the porous gold material. The monolayers were found to be electrochemically active by cyclic and square wave voltammetry. It was found that 24 h HNO3 treatment gave a 12 400× surface enlargement and resulted in a surface area of 14.2 m2/g, whereas 72 h HNO3 treatment resulted in a 6900× surface enlargement and a surface area of 8.2 m2/g. In addition, the enzyme acetylcholine esterase was immobilized on the different porous gold surfaces to demonstrate biocompatibility of the porous gold material. Kinetic parameters and the amount of the immobilized acetylcholine esterase were determined.

AB - We report a method for the fabrication of a free-standing porous gold material with a high surface area and well-defined, tunable pore morphology. Porous gold is formed via a simple procedure that involves acidic treatment of a commercially available complex white gold alloy. We used SEM and AFM techniques to characterize the surface morphology, size, and shape of the mesopores as well as the surface roughness of the prepared porous gold samples. Formation of self-assembled monolayers of a flavin sulfide on the gold surface was used to estimate the total surface area of the porous gold material. The monolayers were found to be electrochemically active by cyclic and square wave voltammetry. It was found that 24 h HNO3 treatment gave a 12 400× surface enlargement and resulted in a surface area of 14.2 m2/g, whereas 72 h HNO3 treatment resulted in a 6900× surface enlargement and a surface area of 8.2 m2/g. In addition, the enzyme acetylcholine esterase was immobilized on the different porous gold surfaces to demonstrate biocompatibility of the porous gold material. Kinetic parameters and the amount of the immobilized acetylcholine esterase were determined.

UR - http://www.scopus.com/inward/record.url?scp=34547959954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547959954&partnerID=8YFLogxK

U2 - 10.1021/cm070238n

DO - 10.1021/cm070238n

M3 - Article

VL - 19

SP - 3902

EP - 3911

JO - Chemistry of Materials

JF - Chemistry of Materials

SN - 0897-4756

IS - 16

ER -