Abstract
We investigated if prenatal exposures to tobacco smoke lead to changes in mitochondrial DNA content (mtDNA) in cord serum and adversely affect newborns’ health. Umbilical cord serum cotinine levels were used to determine in utero exposure to smoking. Cord serum mtDNA was measured by quantitative polymerase chain reaction analysis of the genes coding for cytochrome c oxidase1 (MT-CO1) and cytochrome c oxidase2 (MT-CO2). Log transformed levels of mtDNA coding for MT-CO1 and MT-CO2 were significantly higher among infants of active smokers with higher serum level of cotinine (p < 0.05) and inversely associated with gestational age (p = 0.08; p = 0.02). Structural equation modeling results confirmed a positive association between cotinine and MT-CO1 and2 (p < 0.01) and inverse associations with gestational age (p = 0.02) and IGF-1 (p < 0.01). We identified a dose-dependent increase in the level of MT-CO1 and MT-CO2 associated to increased cord serum cotinine and decreased gestational age.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 52-67 |
| Number of pages | 16 |
| Journal | International Journal of Environmental Health Research |
| Volume | 27 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2 2017 |
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Keywords
- gestational age
- maternal smoking
- mitochondrial DNA content
- molecular biomarkers in cord serum
- MT-CO1
- MT-CO2
- Prenatal exposure
ASJC Scopus subject areas
- Pollution
- Public Health, Environmental and Occupational Health
- Health, Toxicology and Mutagenesis
Cite this
Prenatal exposure to tobacco smoke leads to increased mitochondrial DNA content in umbilical cord serum associated to reduced gestational age. / Pirini, Francesca; Goldman, Lynn R.; Soudry, Ethan; Halden, Rolf; Witter, Frank; Sidransky, David; Guerrero-Preston, Rafael.
In: International Journal of Environmental Health Research, Vol. 27, No. 1, 02.01.2017, p. 52-67.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Prenatal exposure to tobacco smoke leads to increased mitochondrial DNA content in umbilical cord serum associated to reduced gestational age
AU - Pirini, Francesca
AU - Goldman, Lynn R.
AU - Soudry, Ethan
AU - Halden, Rolf
AU - Witter, Frank
AU - Sidransky, David
AU - Guerrero-Preston, Rafael
PY - 2017/1/2
Y1 - 2017/1/2
N2 - We investigated if prenatal exposures to tobacco smoke lead to changes in mitochondrial DNA content (mtDNA) in cord serum and adversely affect newborns’ health. Umbilical cord serum cotinine levels were used to determine in utero exposure to smoking. Cord serum mtDNA was measured by quantitative polymerase chain reaction analysis of the genes coding for cytochrome c oxidase1 (MT-CO1) and cytochrome c oxidase2 (MT-CO2). Log transformed levels of mtDNA coding for MT-CO1 and MT-CO2 were significantly higher among infants of active smokers with higher serum level of cotinine (p < 0.05) and inversely associated with gestational age (p = 0.08; p = 0.02). Structural equation modeling results confirmed a positive association between cotinine and MT-CO1 and2 (p < 0.01) and inverse associations with gestational age (p = 0.02) and IGF-1 (p < 0.01). We identified a dose-dependent increase in the level of MT-CO1 and MT-CO2 associated to increased cord serum cotinine and decreased gestational age.
AB - We investigated if prenatal exposures to tobacco smoke lead to changes in mitochondrial DNA content (mtDNA) in cord serum and adversely affect newborns’ health. Umbilical cord serum cotinine levels were used to determine in utero exposure to smoking. Cord serum mtDNA was measured by quantitative polymerase chain reaction analysis of the genes coding for cytochrome c oxidase1 (MT-CO1) and cytochrome c oxidase2 (MT-CO2). Log transformed levels of mtDNA coding for MT-CO1 and MT-CO2 were significantly higher among infants of active smokers with higher serum level of cotinine (p < 0.05) and inversely associated with gestational age (p = 0.08; p = 0.02). Structural equation modeling results confirmed a positive association between cotinine and MT-CO1 and2 (p < 0.01) and inverse associations with gestational age (p = 0.02) and IGF-1 (p < 0.01). We identified a dose-dependent increase in the level of MT-CO1 and MT-CO2 associated to increased cord serum cotinine and decreased gestational age.
KW - gestational age
KW - maternal smoking
KW - mitochondrial DNA content
KW - molecular biomarkers in cord serum
KW - MT-CO1
KW - MT-CO2
KW - Prenatal exposure
UR - http://www.scopus.com/inward/record.url?scp=85008250405&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85008250405&partnerID=8YFLogxK
U2 - 10.1080/09603123.2016.1268677
DO - 10.1080/09603123.2016.1268677
M3 - Article
C2 - 28002977
AN - SCOPUS:85008250405
VL - 27
SP - 52
EP - 67
JO - International Journal of Environmental Health Research
JF - International Journal of Environmental Health Research
SN - 0960-3123
IS - 1
ER -