Predicting column performance in displacement chromatography from high throughput screening batch experiments

Kaushal Rege, Nihal Tugcu, Steven M. Cramer

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A novel high throughput screening (HTS) technique has been recently developed for displacer discovery. In this article, the multicomponent steric mass action (SMA) model is used to determine column performance in displacement chromatography from batch HTS results. The multicomponent isotherm is first used to predict the displacer concentration in the batch HTS experiments without assaying for the displacer. This information is then employed to determine the single component dynamic affinities of the displacer and the protein and to predict displacement efficacy under column conditions. The model is used to predict the column displacement of horse heart cytochrome-C using N-α-benzoyl arginine ethyl ester as the displacer based on batch HTS results.

Original languageEnglish (US)
Pages (from-to)1499-1517
Number of pages19
JournalSeparation Science and Technology
Volume38
Issue number7
DOIs
StatePublished - May 17 2003
Externally publishedYes

Keywords

  • Displacement chromatography
  • High throughput screening
  • Ion exchange
  • Steric mass action isotherm

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Process Chemistry and Technology
  • Filtration and Separation

Fingerprint Dive into the research topics of 'Predicting column performance in displacement chromatography from high throughput screening batch experiments'. Together they form a unique fingerprint.

  • Cite this