Predicting column performance in displacement chromatography from high throughput screening batch experiments

Kaushal Rege, Nihal Tugcu, Steven M. Cramer

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A novel high throughput screening (HTS) technique has been recently developed for displacer discovery. In this article, the multicomponent steric mass action (SMA) model is used to determine column performance in displacement chromatography from batch HTS results. The multicomponent isotherm is first used to predict the displacer concentration in the batch HTS experiments without assaying for the displacer. This information is then employed to determine the single component dynamic affinities of the displacer and the protein and to predict displacement efficacy under column conditions. The model is used to predict the column displacement of horse heart cytochrome-C using N-α-benzoyl arginine ethyl ester as the displacer based on batch HTS results.

Original languageEnglish (US)
Pages (from-to)1499-1517
Number of pages19
JournalSeparation Science and Technology
Volume38
Issue number7
DOIs
StatePublished - 2003
Externally publishedYes

Fingerprint

Chromatography
Screening
Throughput
Experiments
Proteins
Arginine
Cytochromes
Isotherms
Esters

Keywords

  • Displacement chromatography
  • High throughput screening
  • Ion exchange
  • Steric mass action isotherm

ASJC Scopus subject areas

  • Chemistry(all)
  • Process Chemistry and Technology
  • Chemical Engineering(all)
  • Filtration and Separation

Cite this

Predicting column performance in displacement chromatography from high throughput screening batch experiments. / Rege, Kaushal; Tugcu, Nihal; Cramer, Steven M.

In: Separation Science and Technology, Vol. 38, No. 7, 2003, p. 1499-1517.

Research output: Contribution to journalArticle

@article{7a4e4d88bf734d5a9880665c2c202d8d,
title = "Predicting column performance in displacement chromatography from high throughput screening batch experiments",
abstract = "A novel high throughput screening (HTS) technique has been recently developed for displacer discovery. In this article, the multicomponent steric mass action (SMA) model is used to determine column performance in displacement chromatography from batch HTS results. The multicomponent isotherm is first used to predict the displacer concentration in the batch HTS experiments without assaying for the displacer. This information is then employed to determine the single component dynamic affinities of the displacer and the protein and to predict displacement efficacy under column conditions. The model is used to predict the column displacement of horse heart cytochrome-C using N-α-benzoyl arginine ethyl ester as the displacer based on batch HTS results.",
keywords = "Displacement chromatography, High throughput screening, Ion exchange, Steric mass action isotherm",
author = "Kaushal Rege and Nihal Tugcu and Cramer, {Steven M.}",
year = "2003",
doi = "10.1081/SS-120019089",
language = "English (US)",
volume = "38",
pages = "1499--1517",
journal = "Separation Science and Technology (Philadelphia)",
issn = "0149-6395",
publisher = "Taylor and Francis Ltd.",
number = "7",

}

TY - JOUR

T1 - Predicting column performance in displacement chromatography from high throughput screening batch experiments

AU - Rege, Kaushal

AU - Tugcu, Nihal

AU - Cramer, Steven M.

PY - 2003

Y1 - 2003

N2 - A novel high throughput screening (HTS) technique has been recently developed for displacer discovery. In this article, the multicomponent steric mass action (SMA) model is used to determine column performance in displacement chromatography from batch HTS results. The multicomponent isotherm is first used to predict the displacer concentration in the batch HTS experiments without assaying for the displacer. This information is then employed to determine the single component dynamic affinities of the displacer and the protein and to predict displacement efficacy under column conditions. The model is used to predict the column displacement of horse heart cytochrome-C using N-α-benzoyl arginine ethyl ester as the displacer based on batch HTS results.

AB - A novel high throughput screening (HTS) technique has been recently developed for displacer discovery. In this article, the multicomponent steric mass action (SMA) model is used to determine column performance in displacement chromatography from batch HTS results. The multicomponent isotherm is first used to predict the displacer concentration in the batch HTS experiments without assaying for the displacer. This information is then employed to determine the single component dynamic affinities of the displacer and the protein and to predict displacement efficacy under column conditions. The model is used to predict the column displacement of horse heart cytochrome-C using N-α-benzoyl arginine ethyl ester as the displacer based on batch HTS results.

KW - Displacement chromatography

KW - High throughput screening

KW - Ion exchange

KW - Steric mass action isotherm

UR - http://www.scopus.com/inward/record.url?scp=0037625875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037625875&partnerID=8YFLogxK

U2 - 10.1081/SS-120019089

DO - 10.1081/SS-120019089

M3 - Article

AN - SCOPUS:0037625875

VL - 38

SP - 1499

EP - 1517

JO - Separation Science and Technology (Philadelphia)

JF - Separation Science and Technology (Philadelphia)

SN - 0149-6395

IS - 7

ER -