Precise automated control of fluid volumes inside glass capillaries

Marco J. Daoura, Deirdre Meldrum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A precise automated apparatus for controlling the position of small fluid sample volumes inside glass capillaries has been developed as part of an automated system for handling submicroliter reaction volumes for genomic analysis [1], [2]. The apparatus uses a linear charge-coupled device (CCD) array, dc motor, pneumatic pump, feedback controller, and a digital signal processor (DSP) to detect the menisci and control in real-time the position of fluid in a clear glass capillary with a resolution of 50 μm. This information is used to infer fluid volumes and control aspiration and dispensing to within 15 nanoliters depending on the inner diameter of the capillary. A computer simulation was developed to determine the effect of thick- and thin-walled capillaries on the detected signals. This simulation showed that, with a geometry appropriate for the overall automated system, a capillary with an inner diameter greater than 0.6 times the outer diameter is optimal to detect the presence of fluid inside the capillary. Thicker walled glass capillaries may be employed if a focusing lens is placed between the light source and the capillary. This result was verified experimentally.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalJournal of Microelectromechanical Systems
Volume8
Issue number1
DOIs
StatePublished - Mar 1999
Externally publishedYes

Fingerprint

Glass
Fluids
Digital signal processors
Charge coupled devices
Pneumatics
Light sources
Lenses
Pumps
Feedback
Controllers
Geometry
Computer simulation

Keywords

  • Automation
  • Biotechnology
  • Detection
  • Fluidics
  • Integrated systems
  • Sample processing
  • Submicroliter

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering

Cite this

Precise automated control of fluid volumes inside glass capillaries. / Daoura, Marco J.; Meldrum, Deirdre.

In: Journal of Microelectromechanical Systems, Vol. 8, No. 1, 03.1999, p. 71-77.

Research output: Contribution to journalArticle

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