Poxvirus proteomics and virus-host protein interactions

Kim Van Vlie, Mohamed R. Mohamed, Leiliang Zhang, Nancy Yaneth Villa, Steven J. Werden, Jia Liu, Grant McFadden

Research output: Contribution to journalReview articlepeer-review

36 Scopus citations

Abstract

Studies of the functional proteins encoded by the poxvirus genome provide information about the composition of the virus as well as individual virusvirus protein and virus-host protein interactions, which provides insight into viral pathogenesis and drug discovery. Widely used proteomic techniques to identify and characterize specific protein-protein interactions include yeast two-hybrid studies and coimmunoprecipitations. Recently, various mass spectrometry techniques have been employed to identify viral protein components of larger complexes. These methods, combined with structural studies, can provide new information about the putative functions of viral proteins as well as insights into virus-host interaction dynamics. For viral proteins of unknown function, identification of either viral or host binding partners provides clues about their putative function. In this review, we discuss poxvirus proteomics, including the use of proteomic methodologies to identify viral components and virus-host protein interactions. High-throughput global protein expression studies using protein chip technology as well as new methods for validating putative protein-protein interactions are also discussed.

Original languageEnglish (US)
Pages (from-to)730-749
Number of pages20
JournalMicrobiology and Molecular Biology Reviews
Volume73
Issue number4
DOIs
StatePublished - Dec 2009

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

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