Poxvirus Infection Rapidly Activates Tyrosine Kinase Signal Transduction

Jennefer Masters, Anna A. Hinek, Shahab Uddin, Leonidas C. Platanias, Wei Zeng, Douglas McFadden, Eleanor N. Fish

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Viruses have evolved a number of strategies to gain entry and replicate in host target cells that, for human immunodeficiency virus (HIV) and the poxvirus, myxoma virus, involve appropriating chemokine receptors. In this report we demonstrate that activation of multiple intracellular tyrosine phosphorylation events rapidly ensues following virus adsorption to NIH 3T3.CD4.CCR5 cells and affects the ultimate level of myxoma virus replication. UV-inactivated myxoma virus induces the rapid phosphorylation of CCR5 on tyrosine residues, the association of CCR5 with Jaks and p56lck, and their phosphorylation-activation within minutes of virus adsorption. Additionally, we provide evidence for myxoma virus-inducible signal transducers and activators of transcription (Stat) and insulin receptor substrate (IRS) activation. In contrast to CCR5 activation effected by HIV Env protein, these myxoma virus-inducible phosphorylation events are not sensitive to pertussis toxin treatment. Moreover, in cells that are non-permissive for myxoma virus infection, we provide evidence that myxoma virus fails to invoke this tyrosine phosphorylation cascade. Consistent with the observation that infection of CCR5-expressing cells is blocked by herbimycin A and the Jak 2 inhibitor, tyrophostin AG490, we infer that viral infectivity may be dependent on non-G-protein-coupled signal transduction pathways triggered by the infecting myxoma virus particle. This provides a novel post-binding mechanism by which viruses can co-opt a cellular receptor to permit productive virus infection.

Original languageEnglish (US)
Pages (from-to)48371-48375
Number of pages5
JournalJournal of Biological Chemistry
Volume276
Issue number51
DOIs
StatePublished - Dec 21 2001
Externally publishedYes

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Poxviridae Infections
Myxoma virus
Signal transduction
Viruses
Protein-Tyrosine Kinases
Signal Transduction
Phosphorylation
Tyrosine
Virus Diseases
Adsorption
Chemical activation
Human Immunodeficiency Virus env Gene Products
Poxviridae
Chemokine Receptors
Insulin Receptor
Pertussis Toxin
Virus Replication
Transducers
Virion
HIV

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Masters, J., Hinek, A. A., Uddin, S., Platanias, L. C., Zeng, W., McFadden, D., & Fish, E. N. (2001). Poxvirus Infection Rapidly Activates Tyrosine Kinase Signal Transduction. Journal of Biological Chemistry, 276(51), 48371-48375. https://doi.org/10.1074/jbc.M108019200

Poxvirus Infection Rapidly Activates Tyrosine Kinase Signal Transduction. / Masters, Jennefer; Hinek, Anna A.; Uddin, Shahab; Platanias, Leonidas C.; Zeng, Wei; McFadden, Douglas; Fish, Eleanor N.

In: Journal of Biological Chemistry, Vol. 276, No. 51, 21.12.2001, p. 48371-48375.

Research output: Contribution to journalArticle

Masters, J, Hinek, AA, Uddin, S, Platanias, LC, Zeng, W, McFadden, D & Fish, EN 2001, 'Poxvirus Infection Rapidly Activates Tyrosine Kinase Signal Transduction', Journal of Biological Chemistry, vol. 276, no. 51, pp. 48371-48375. https://doi.org/10.1074/jbc.M108019200
Masters, Jennefer ; Hinek, Anna A. ; Uddin, Shahab ; Platanias, Leonidas C. ; Zeng, Wei ; McFadden, Douglas ; Fish, Eleanor N. / Poxvirus Infection Rapidly Activates Tyrosine Kinase Signal Transduction. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 51. pp. 48371-48375.
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