Abstract
Background: Apolipoprotein A-II (apoA-II) is the second most abundant protein in high-density lipoprotein particles. However, it exists in plasma in multiple forms. The effect of diabetes on apoA-II proteoforms is not known. Objective: Our objective was to characterize plasma apoA-II proteoforms in participants with and without type 2 diabetes. Methods: Using a novel mass spectrometric immunoassay, the relative abundance of apoA-II proteoforms was examined in plasma of 30 participants with type 2 diabetes and 25 participants without diabetes. Results: Six apoA-II proteoforms (monomer, truncated TQ monomer, truncated Q monomer, dimer, truncated Q dimer, and truncated 2Qs dimer) and their oxidized proteoforms were identified. The ratios of oxidized monomer and all oxidized proteoforms to the native apoA-II were significantly greater in the diabetic group (P = .004 and P = .005, respectively) compared with the nondiabetic group. Conclusion: The relative abundance of oxidized apoA-II is significantly increased in type 2 diabetes.
| Original language | English (US) |
|---|---|
| Journal | Journal of Clinical Lipidology |
| DOIs | |
| State | Accepted/In press - Nov 3 2015 |
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Keywords
- Apolipoprotein A-II
- Diabetes
- Mass spectrometry
- Oxidations
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Endocrinology, Diabetes and Metabolism
- Internal Medicine
- Nutrition and Dietetics
Cite this
Posttranslational modifications of apolipoprotein A-II proteoforms in type 2 diabetes. / Azizkhanian, Ida; Trenchevska, Olgica; Bashawri, Yara; Hu, Jiaqi; Koska, Juraj; Reaven, Peter D.; Nelson, Randall W.; Nedelkov, Dobrin; Yassine, Hussein N.
In: Journal of Clinical Lipidology, 03.11.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Posttranslational modifications of apolipoprotein A-II proteoforms in type 2 diabetes
AU - Azizkhanian, Ida
AU - Trenchevska, Olgica
AU - Bashawri, Yara
AU - Hu, Jiaqi
AU - Koska, Juraj
AU - Reaven, Peter D.
AU - Nelson, Randall W.
AU - Nedelkov, Dobrin
AU - Yassine, Hussein N.
PY - 2015/11/3
Y1 - 2015/11/3
N2 - Background: Apolipoprotein A-II (apoA-II) is the second most abundant protein in high-density lipoprotein particles. However, it exists in plasma in multiple forms. The effect of diabetes on apoA-II proteoforms is not known. Objective: Our objective was to characterize plasma apoA-II proteoforms in participants with and without type 2 diabetes. Methods: Using a novel mass spectrometric immunoassay, the relative abundance of apoA-II proteoforms was examined in plasma of 30 participants with type 2 diabetes and 25 participants without diabetes. Results: Six apoA-II proteoforms (monomer, truncated TQ monomer, truncated Q monomer, dimer, truncated Q dimer, and truncated 2Qs dimer) and their oxidized proteoforms were identified. The ratios of oxidized monomer and all oxidized proteoforms to the native apoA-II were significantly greater in the diabetic group (P = .004 and P = .005, respectively) compared with the nondiabetic group. Conclusion: The relative abundance of oxidized apoA-II is significantly increased in type 2 diabetes.
AB - Background: Apolipoprotein A-II (apoA-II) is the second most abundant protein in high-density lipoprotein particles. However, it exists in plasma in multiple forms. The effect of diabetes on apoA-II proteoforms is not known. Objective: Our objective was to characterize plasma apoA-II proteoforms in participants with and without type 2 diabetes. Methods: Using a novel mass spectrometric immunoassay, the relative abundance of apoA-II proteoforms was examined in plasma of 30 participants with type 2 diabetes and 25 participants without diabetes. Results: Six apoA-II proteoforms (monomer, truncated TQ monomer, truncated Q monomer, dimer, truncated Q dimer, and truncated 2Qs dimer) and their oxidized proteoforms were identified. The ratios of oxidized monomer and all oxidized proteoforms to the native apoA-II were significantly greater in the diabetic group (P = .004 and P = .005, respectively) compared with the nondiabetic group. Conclusion: The relative abundance of oxidized apoA-II is significantly increased in type 2 diabetes.
KW - Apolipoprotein A-II
KW - Diabetes
KW - Mass spectrometry
KW - Oxidations
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UR - http://www.scopus.com/inward/citedby.url?scp=84963969074&partnerID=8YFLogxK
U2 - 10.1016/j.jacl.2016.03.001
DO - 10.1016/j.jacl.2016.03.001
M3 - Article
JO - Journal of Clinical Lipidology
JF - Journal of Clinical Lipidology
SN - 1933-2874
ER -