Postsynaptic cutaneous vasodilation and sweating: influence of adiposity and hydration status

Matthew A. Tucker, Aaron R. Caldwell, Cory L. Butts, Forrest B. Robinson, Stavros A. Kavouras, Brendon P. McDermott, Tyrone A. Washington, Ronna C. Turner, Matthew S. Ganio

Research output: Contribution to journalArticle

Abstract

Introduction: Obesity and hypohydration independently affect postsynaptic endothelial function, but it is unknown if hypohydration affects lean and obese individuals differently. Purpose: To examine the effect of hypohydration on postsynaptic cutaneous vasodilation and sweating in men with high and low adiposity (HI- and LO-BF, respectively). Methods: Ten males with LO-BF and ten with HI-BF were instrumented for forearm microdialysis when euhydrated and hypohydrated. Changes in cutaneous vascular conductance (CVC) with intradermal infusion of sodium nitroprusside (SNP) and methacholine chloride (MCh) were assessed. Local sweat rate (LSR) was simultaneously assessed at the MCh site. At the end of the last dose, maximal CVC was elicited by delivering a maximal dose of SNP for 30 min to both sites with simultaneous local heating at the SNP site. The concentration of drug needed to elicit 50% of the maximal response (EC50) was compared between groups and hydration conditions. Results: When euhydrated, EC50 of MCh-induced CVC was not different between LO- vs. HI-BF [− 3.04 ± 0.12 vs. − 2.98 ± 0.19 log (MCh) M, P = 0.841]. EC50 of SNP-induced CVC was higher in euhydrated HI- vs. LO-BF (− 1.74 ± 0.17 vs. − 2.13 ± 0.06 log (SNP) M, P = 0.034). Within each group, hydration status did not change MCh- or SNP-induced CVC (P > 0.05). LSR was not different between groups or hydration condition (P > 0.05). Conclusions: These data suggest reduced sensitivity of endothelium-independent vasodilation in individuals with high adiposity when euhydrated. However, hypohydration does not affect cutaneous vasodilation or local sweat rate differently between individuals with low or high adiposity.

Original languageEnglish (US)
Pages (from-to)1703-1713
Number of pages11
JournalEuropean Journal of Applied Physiology
Volume118
Issue number8
DOIs
StatePublished - Aug 1 2018
Externally publishedYes

Fingerprint

Sweating
Adiposity
Vasodilation
Nitroprusside
Methacholine Chloride
Blood Vessels
Skin
Sweat
Microdialysis
Forearm
Sodium Chloride
Heating
Endothelium
Obesity
Pharmaceutical Preparations

Keywords

  • Dehydration
  • Microvascular dysfunction
  • Skin blood flow
  • Thermoregulation

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Public Health, Environmental and Occupational Health
  • Physiology (medical)

Cite this

Tucker, M. A., Caldwell, A. R., Butts, C. L., Robinson, F. B., Kavouras, S. A., McDermott, B. P., ... Ganio, M. S. (2018). Postsynaptic cutaneous vasodilation and sweating: influence of adiposity and hydration status. European Journal of Applied Physiology, 118(8), 1703-1713. https://doi.org/10.1007/s00421-018-3902-3

Postsynaptic cutaneous vasodilation and sweating : influence of adiposity and hydration status. / Tucker, Matthew A.; Caldwell, Aaron R.; Butts, Cory L.; Robinson, Forrest B.; Kavouras, Stavros A.; McDermott, Brendon P.; Washington, Tyrone A.; Turner, Ronna C.; Ganio, Matthew S.

In: European Journal of Applied Physiology, Vol. 118, No. 8, 01.08.2018, p. 1703-1713.

Research output: Contribution to journalArticle

Tucker, MA, Caldwell, AR, Butts, CL, Robinson, FB, Kavouras, SA, McDermott, BP, Washington, TA, Turner, RC & Ganio, MS 2018, 'Postsynaptic cutaneous vasodilation and sweating: influence of adiposity and hydration status', European Journal of Applied Physiology, vol. 118, no. 8, pp. 1703-1713. https://doi.org/10.1007/s00421-018-3902-3
Tucker, Matthew A. ; Caldwell, Aaron R. ; Butts, Cory L. ; Robinson, Forrest B. ; Kavouras, Stavros A. ; McDermott, Brendon P. ; Washington, Tyrone A. ; Turner, Ronna C. ; Ganio, Matthew S. / Postsynaptic cutaneous vasodilation and sweating : influence of adiposity and hydration status. In: European Journal of Applied Physiology. 2018 ; Vol. 118, No. 8. pp. 1703-1713.
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abstract = "Introduction: Obesity and hypohydration independently affect postsynaptic endothelial function, but it is unknown if hypohydration affects lean and obese individuals differently. Purpose: To examine the effect of hypohydration on postsynaptic cutaneous vasodilation and sweating in men with high and low adiposity (HI- and LO-BF, respectively). Methods: Ten males with LO-BF and ten with HI-BF were instrumented for forearm microdialysis when euhydrated and hypohydrated. Changes in cutaneous vascular conductance (CVC) with intradermal infusion of sodium nitroprusside (SNP) and methacholine chloride (MCh) were assessed. Local sweat rate (LSR) was simultaneously assessed at the MCh site. At the end of the last dose, maximal CVC was elicited by delivering a maximal dose of SNP for 30 min to both sites with simultaneous local heating at the SNP site. The concentration of drug needed to elicit 50{\%} of the maximal response (EC50) was compared between groups and hydration conditions. Results: When euhydrated, EC50 of MCh-induced CVC was not different between LO- vs. HI-BF [− 3.04 ± 0.12 vs. − 2.98 ± 0.19 log (MCh) M, P = 0.841]. EC50 of SNP-induced CVC was higher in euhydrated HI- vs. LO-BF (− 1.74 ± 0.17 vs. − 2.13 ± 0.06 log (SNP) M, P = 0.034). Within each group, hydration status did not change MCh- or SNP-induced CVC (P > 0.05). LSR was not different between groups or hydration condition (P > 0.05). Conclusions: These data suggest reduced sensitivity of endothelium-independent vasodilation in individuals with high adiposity when euhydrated. However, hypohydration does not affect cutaneous vasodilation or local sweat rate differently between individuals with low or high adiposity.",
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T1 - Postsynaptic cutaneous vasodilation and sweating

T2 - influence of adiposity and hydration status

AU - Tucker, Matthew A.

AU - Caldwell, Aaron R.

AU - Butts, Cory L.

AU - Robinson, Forrest B.

AU - Kavouras, Stavros A.

AU - McDermott, Brendon P.

AU - Washington, Tyrone A.

AU - Turner, Ronna C.

AU - Ganio, Matthew S.

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N2 - Introduction: Obesity and hypohydration independently affect postsynaptic endothelial function, but it is unknown if hypohydration affects lean and obese individuals differently. Purpose: To examine the effect of hypohydration on postsynaptic cutaneous vasodilation and sweating in men with high and low adiposity (HI- and LO-BF, respectively). Methods: Ten males with LO-BF and ten with HI-BF were instrumented for forearm microdialysis when euhydrated and hypohydrated. Changes in cutaneous vascular conductance (CVC) with intradermal infusion of sodium nitroprusside (SNP) and methacholine chloride (MCh) were assessed. Local sweat rate (LSR) was simultaneously assessed at the MCh site. At the end of the last dose, maximal CVC was elicited by delivering a maximal dose of SNP for 30 min to both sites with simultaneous local heating at the SNP site. The concentration of drug needed to elicit 50% of the maximal response (EC50) was compared between groups and hydration conditions. Results: When euhydrated, EC50 of MCh-induced CVC was not different between LO- vs. HI-BF [− 3.04 ± 0.12 vs. − 2.98 ± 0.19 log (MCh) M, P = 0.841]. EC50 of SNP-induced CVC was higher in euhydrated HI- vs. LO-BF (− 1.74 ± 0.17 vs. − 2.13 ± 0.06 log (SNP) M, P = 0.034). Within each group, hydration status did not change MCh- or SNP-induced CVC (P > 0.05). LSR was not different between groups or hydration condition (P > 0.05). Conclusions: These data suggest reduced sensitivity of endothelium-independent vasodilation in individuals with high adiposity when euhydrated. However, hypohydration does not affect cutaneous vasodilation or local sweat rate differently between individuals with low or high adiposity.

AB - Introduction: Obesity and hypohydration independently affect postsynaptic endothelial function, but it is unknown if hypohydration affects lean and obese individuals differently. Purpose: To examine the effect of hypohydration on postsynaptic cutaneous vasodilation and sweating in men with high and low adiposity (HI- and LO-BF, respectively). Methods: Ten males with LO-BF and ten with HI-BF were instrumented for forearm microdialysis when euhydrated and hypohydrated. Changes in cutaneous vascular conductance (CVC) with intradermal infusion of sodium nitroprusside (SNP) and methacholine chloride (MCh) were assessed. Local sweat rate (LSR) was simultaneously assessed at the MCh site. At the end of the last dose, maximal CVC was elicited by delivering a maximal dose of SNP for 30 min to both sites with simultaneous local heating at the SNP site. The concentration of drug needed to elicit 50% of the maximal response (EC50) was compared between groups and hydration conditions. Results: When euhydrated, EC50 of MCh-induced CVC was not different between LO- vs. HI-BF [− 3.04 ± 0.12 vs. − 2.98 ± 0.19 log (MCh) M, P = 0.841]. EC50 of SNP-induced CVC was higher in euhydrated HI- vs. LO-BF (− 1.74 ± 0.17 vs. − 2.13 ± 0.06 log (SNP) M, P = 0.034). Within each group, hydration status did not change MCh- or SNP-induced CVC (P > 0.05). LSR was not different between groups or hydration condition (P > 0.05). Conclusions: These data suggest reduced sensitivity of endothelium-independent vasodilation in individuals with high adiposity when euhydrated. However, hypohydration does not affect cutaneous vasodilation or local sweat rate differently between individuals with low or high adiposity.

KW - Dehydration

KW - Microvascular dysfunction

KW - Skin blood flow

KW - Thermoregulation

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