TY - JOUR
T1 - Postfertilization growth of microvilli in the sea urchin egg
T2 - New views from eggs that have been quick-frozen, freeze-fractured, and deeply etched
AU - Chandler, Douglas E.
AU - Heuser, John
N1 - Funding Information:
We thank Louise Evans for her excellent technical assistance and Dr. Parris Kidd for many helpful discussionsa nd for making available his unpublished results. This study was carried out during tenure of a postdoctoral fellowship from the Pharmaceutical Manufacturer’s Association Foundation (to D.E.C.) and was suported by grants (to J.E.H.) from the USPHS (NS 11979)a nd the Muscular Dystrophy Association.
PY - 1981/3
Y1 - 1981/3
N2 - We have used quick-freezing, freeze-fracture, and deep etching to study postfertilization growth of microvilli in the sea urchin, Strongylocentrotus purpuratus. Between 1 and 2 min postinsemination, we observed rapid growth of numerous finger-like microvilli to about 0.7 μm in length. Subsequently, groups of two to four microvilli became raised off the egg surface on broad cytoplasmic bases. Finally, by 5 min postinsemination, these bases had become interconnected by thin sheets of cytoplasm to form a continuous network of ruffles across the egg surface. Corresponding thin sections of quick-frozen and freeze-substituted eggs showed that these microvilli contained networks of microfilaments. The finger-like processes had bundles of parallel microfilaments that ran their entire length, while the ruffles of cytoplasm contained sheets of crisscrossed microfilaments. These observations support the hypothesis that microvillar growth results from formation of new microfilament networks.
AB - We have used quick-freezing, freeze-fracture, and deep etching to study postfertilization growth of microvilli in the sea urchin, Strongylocentrotus purpuratus. Between 1 and 2 min postinsemination, we observed rapid growth of numerous finger-like microvilli to about 0.7 μm in length. Subsequently, groups of two to four microvilli became raised off the egg surface on broad cytoplasmic bases. Finally, by 5 min postinsemination, these bases had become interconnected by thin sheets of cytoplasm to form a continuous network of ruffles across the egg surface. Corresponding thin sections of quick-frozen and freeze-substituted eggs showed that these microvilli contained networks of microfilaments. The finger-like processes had bundles of parallel microfilaments that ran their entire length, while the ruffles of cytoplasm contained sheets of crisscrossed microfilaments. These observations support the hypothesis that microvillar growth results from formation of new microfilament networks.
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U2 - 10.1016/0012-1606(81)90463-2
DO - 10.1016/0012-1606(81)90463-2
M3 - Article
C2 - 7227649
AN - SCOPUS:0019412422
SN - 0012-1606
VL - 82
SP - 393
EP - 400
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -