Abstract
The success of diffraction experiments from weakly scattering samples strongly depends on achieving an optimal signal-to-noise ratio. This is particularly important in single-particle imaging experiments where diffraction signals are typically very weak and the experiments are often accompanied by significant background scattering. A simple way to tremendously reduce background scattering by placing an aperture downstream of the sample has been developed and its application in a single-particle X-ray imaging experiment at FLASH is demonstrated. Using the concept of a post-sample aperture it was possible to reduce the background scattering levels by two orders of magnitude.Diffraction experiments with weakly scattering samples often suffer from a low signal-to-noise ratio due to unwanted background scatter. Improving the signal-to-noise ratio for single-particle imaging experiments is particularly important as the diffraction signal is very weak. Here, a simple way to minimize the background scattering by placing an aperture downstream of the sample is demonstrated.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1296-1298 |
| Number of pages | 3 |
| Journal | Journal of Synchrotron Radiation |
| Volume | 24 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 1 2017 |
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Keywords
- aperture
- background scattering
- coherent diffractive imaging
- signal-to-noise ratio
- single-particle imaging
- X-ray diffraction
ASJC Scopus subject areas
- Radiation
- Nuclear and High Energy Physics
- Instrumentation
Cite this
Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers. / Wiedorn, Max O.; Awel, Salah; Morgan, Andrew J.; Barthelmess, Miriam; Bean, Richard; Beyerlein, Kenneth R.; Chavas, Leonard M.G.; Eckerskorn, Niko; Fleckenstein, Holger; Heymann, Michael; Horke, Daniel A.; Knoška, Juraj; Mariani, Valerio; Oberthür, Dominik; Roth, Nils; Yefanov, Oleksandr; Barty, Anton; Bajt, Saša; Küpper, Jochen; Rode, Andrei V.; Kirian, Richard; Chapman, Henry N.
In: Journal of Synchrotron Radiation, Vol. 24, No. 6, 01.11.2017, p. 1296-1298.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers
AU - Wiedorn, Max O.
AU - Awel, Salah
AU - Morgan, Andrew J.
AU - Barthelmess, Miriam
AU - Bean, Richard
AU - Beyerlein, Kenneth R.
AU - Chavas, Leonard M.G.
AU - Eckerskorn, Niko
AU - Fleckenstein, Holger
AU - Heymann, Michael
AU - Horke, Daniel A.
AU - Knoška, Juraj
AU - Mariani, Valerio
AU - Oberthür, Dominik
AU - Roth, Nils
AU - Yefanov, Oleksandr
AU - Barty, Anton
AU - Bajt, Saša
AU - Küpper, Jochen
AU - Rode, Andrei V.
AU - Kirian, Richard
AU - Chapman, Henry N.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - The success of diffraction experiments from weakly scattering samples strongly depends on achieving an optimal signal-to-noise ratio. This is particularly important in single-particle imaging experiments where diffraction signals are typically very weak and the experiments are often accompanied by significant background scattering. A simple way to tremendously reduce background scattering by placing an aperture downstream of the sample has been developed and its application in a single-particle X-ray imaging experiment at FLASH is demonstrated. Using the concept of a post-sample aperture it was possible to reduce the background scattering levels by two orders of magnitude.Diffraction experiments with weakly scattering samples often suffer from a low signal-to-noise ratio due to unwanted background scatter. Improving the signal-to-noise ratio for single-particle imaging experiments is particularly important as the diffraction signal is very weak. Here, a simple way to minimize the background scattering by placing an aperture downstream of the sample is demonstrated.
AB - The success of diffraction experiments from weakly scattering samples strongly depends on achieving an optimal signal-to-noise ratio. This is particularly important in single-particle imaging experiments where diffraction signals are typically very weak and the experiments are often accompanied by significant background scattering. A simple way to tremendously reduce background scattering by placing an aperture downstream of the sample has been developed and its application in a single-particle X-ray imaging experiment at FLASH is demonstrated. Using the concept of a post-sample aperture it was possible to reduce the background scattering levels by two orders of magnitude.Diffraction experiments with weakly scattering samples often suffer from a low signal-to-noise ratio due to unwanted background scatter. Improving the signal-to-noise ratio for single-particle imaging experiments is particularly important as the diffraction signal is very weak. Here, a simple way to minimize the background scattering by placing an aperture downstream of the sample is demonstrated.
KW - aperture
KW - background scattering
KW - coherent diffractive imaging
KW - signal-to-noise ratio
KW - single-particle imaging
KW - X-ray diffraction
UR - http://www.scopus.com/inward/record.url?scp=85032700501&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85032700501&partnerID=8YFLogxK
U2 - 10.1107/S1600577517011961
DO - 10.1107/S1600577517011961
M3 - Article
C2 - 29091073
AN - SCOPUS:85032700501
VL - 24
SP - 1296
EP - 1298
JO - Journal of Synchrotron Radiation
JF - Journal of Synchrotron Radiation
SN - 0909-0495
IS - 6
ER -