Position of uridine thiation: The identification of minor nucleosides from transfer RNA by mass spectrometry

Sidney M. Hecht; Amrit S. Gupta; Nelson J. Leonard

doi:10.1016/0005-2787(69)90195-6

Position of uridine thiation: The identification of minor nucleosides from transfer RNA by mass spectrometry

Sidney M. Hecht, Amrit S. Gupta, Nelson J. Leonard

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The position of uridine or uracil thiation may be deduced on the basis of mass spectral fragmentation patterns. Unlike other methods of differentiation, neither chemical conversions nor model compounds are required, and the procedure appears to be independent of substituents on the uracil nucleus. The fragmentation pattern of the base moiety of the nucleoside, which involves the formal loss of HCNO(S) from a uracil (or 2-thiouracil), has been observed to be consistent for uracil, 2-thiouracil, 4-thiouracil, 2,4-dithiouracil, and for many substituted uracils and uridines.

Original language	English (US)
Pages (from-to)	444-448
Number of pages	5
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	182
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(69)90195-6
State	Published - Jun 17 1969
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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10.1016/0005-2787(69)90195-6

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title = "Position of uridine thiation: The identification of minor nucleosides from transfer RNA by mass spectrometry",

abstract = "The position of uridine or uracil thiation may be deduced on the basis of mass spectral fragmentation patterns. Unlike other methods of differentiation, neither chemical conversions nor model compounds are required, and the procedure appears to be independent of substituents on the uracil nucleus. The fragmentation pattern of the base moiety of the nucleoside, which involves the formal loss of HCNO(S) from a uracil (or 2-thiouracil), has been observed to be consistent for uracil, 2-thiouracil, 4-thiouracil, 2,4-dithiouracil, and for many substituted uracils and uridines.",

author = "Hecht, {Sidney M.} and Gupta, {Amrit S.} and Leonard, {Nelson J.}",

note = "Funding Information: This work was supported by research grant GM-o5829 from the National Institutes of Health, U.S. Public Health Service and by a National Institutes of Health predoctoral fellowship (1967-1969) to one of us (S.M.H.).",

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doi = "10.1016/0005-2787(69)90195-6",

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T2 - The identification of minor nucleosides from transfer RNA by mass spectrometry

AU - Hecht, Sidney M.

AU - Gupta, Amrit S.

AU - Leonard, Nelson J.

N1 - Funding Information: This work was supported by research grant GM-o5829 from the National Institutes of Health, U.S. Public Health Service and by a National Institutes of Health predoctoral fellowship (1967-1969) to one of us (S.M.H.).

PY - 1969/6/17

Y1 - 1969/6/17

N2 - The position of uridine or uracil thiation may be deduced on the basis of mass spectral fragmentation patterns. Unlike other methods of differentiation, neither chemical conversions nor model compounds are required, and the procedure appears to be independent of substituents on the uracil nucleus. The fragmentation pattern of the base moiety of the nucleoside, which involves the formal loss of HCNO(S) from a uracil (or 2-thiouracil), has been observed to be consistent for uracil, 2-thiouracil, 4-thiouracil, 2,4-dithiouracil, and for many substituted uracils and uridines.

AB - The position of uridine or uracil thiation may be deduced on the basis of mass spectral fragmentation patterns. Unlike other methods of differentiation, neither chemical conversions nor model compounds are required, and the procedure appears to be independent of substituents on the uracil nucleus. The fragmentation pattern of the base moiety of the nucleoside, which involves the formal loss of HCNO(S) from a uracil (or 2-thiouracil), has been observed to be consistent for uracil, 2-thiouracil, 4-thiouracil, 2,4-dithiouracil, and for many substituted uracils and uridines.

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Position of uridine thiation: The identification of minor nucleosides from transfer RNA by mass spectrometry

Abstract

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