Position of uridine thiation: The identification of minor nucleosides from transfer RNA by mass spectrometry

Sidney M. Hecht, Amrit S. Gupta, Nelson J. Leonard

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The position of uridine or uracil thiation may be deduced on the basis of mass spectral fragmentation patterns. Unlike other methods of differentiation, neither chemical conversions nor model compounds are required, and the procedure appears to be independent of substituents on the uracil nucleus. The fragmentation pattern of the base moiety of the nucleoside, which involves the formal loss of HCNO(S) from a uracil (or 2-thiouracil), has been observed to be consistent for uracil, 2-thiouracil, 4-thiouracil, 2,4-dithiouracil, and for many substituted uracils and uridines.

Original languageEnglish (US)
Pages (from-to)444-448
Number of pages5
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume182
Issue number2
DOIs
StatePublished - Jun 17 1969
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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