Porphyromonas gingivalis-induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase

Mariko Naito, Eiko Sakai, Yixin Shi, Hiroshi Ideguchi, Mikio Shoji, Naoya Ohara, Kenji Yamamoto, Koji Nakayama

Research output: Contribution to journalArticle

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Abstract

Evidence from recent epidemiological studies suggests a link between periodontal infections and increased risk of atherosclerosis and related cardiovascular and cerebrovascular events in human subjects. One of the major pathogens of periodontitis, Porphyromonas gingivalis, has the ability to aggregate human platelets in platelet-rich plasma (PRP). Mechanism of P. gingivalis-induced platelet aggregation in PRP was investigated. Proteinase inhibitors toward Arg-gingipain (Rgp) and Lys-gingipain (Kgp) did not suppress P. gingivalis-induced platelet aggregation in PRP, whereas the Rgp inhibitor markedly inhibited P. gingivalis-induced platelet aggregation using washed platelets. Mutant analysis revealed that P. gingivalis-induced platelet aggregation in PRP depended on Rgp-, Kgp- and haemagglutinin A (HagA)-encoding genes that intragenically coded for adhesins such as Hgp44. Hgp44 adhesin on the bacterial cell surface, which was processed by Rgp and Kgp proteinases, was essential for P. gingivalis-induced platelet aggregation in PRP. P. gingivalis cell-reactive IgG in plasma, and FcγRIIa receptor and to a lesser extent GPIbα receptor on platelets were found to be a prerequisite for P. gingivalis-induced platelet aggregation in PRP. These results reveal a novel mechanism of platelet aggregation by P. gingivalis.

Original languageEnglish (US)
Pages (from-to)152-167
Number of pages16
JournalMolecular Microbiology
Volume59
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

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Porphyromonas gingivalis
Platelet Aggregation
Platelet-Rich Plasma
Peptide Hydrolases
Blood Platelets
Bacterial Adhesins
Periodontitis
Hemagglutinins
Epidemiologic Studies
Atherosclerosis
Immunoglobulin G

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Porphyromonas gingivalis-induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase. / Naito, Mariko; Sakai, Eiko; Shi, Yixin; Ideguchi, Hiroshi; Shoji, Mikio; Ohara, Naoya; Yamamoto, Kenji; Nakayama, Koji.

In: Molecular Microbiology, Vol. 59, No. 1, 01.2006, p. 152-167.

Research output: Contribution to journalArticle

Naito, Mariko ; Sakai, Eiko ; Shi, Yixin ; Ideguchi, Hiroshi ; Shoji, Mikio ; Ohara, Naoya ; Yamamoto, Kenji ; Nakayama, Koji. / Porphyromonas gingivalis-induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase. In: Molecular Microbiology. 2006 ; Vol. 59, No. 1. pp. 152-167.
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